Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 205, P. 106114 - 106114
Published: Sept. 1, 2024
Language: Английский
Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 13, 2025
ABSTRACT RNA interference (RNAi) is increasingly used for plant protection against pathogens and pests. However, the traditional delivery method causes tissue damage, affected by environmental factors, faces difficulties in penetrating barriers of cell walls limitations species, ultimately leading to low efficiency. With advances nanotechnology, nanomaterials (NMs) have been identified as effective carriers nucleic acid because their ability operate independently external mechanical forces, prevent degradation bioenzymes, exhibit good biocompatibility, offer high loading capacity. This review summarizes application NM‐mediated RNAi pests, focusing on how different NMs break through plants, pathogens, pests according size, morphology, charge characteristics. Furthermore, we discuss advantages improvement strategies carriers, alongside assessing potential management
Language: Английский
Citations
0
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Sitophilus zeamais is a highly destructive pest of stored grains worldwide. To develop novel RNA insecticides, the double-stranded S. cytochrome c oxidase (dsSzCOXs: dsSzCOXI, dsSzCOXII, and dsSzCOXIII) was successfully expressed in pET28a-BL21(DE3)-RNaseIII- system remarkably downregulated expression level SzCOXs through injection or feeding methods, leading to high mortalities both larvae adults. Under their optimal conditions (isopropyl β-d-thiogalactoside, 0.6 mM), yields dsSzCOXI (at 28 °C for 4 h), dsSzCOXII 37 6 dsSzCOXIII h) were 8.49, 5.80, 6.04 μg/mL respectively. The chitosan-based gene delivery composed dsSzCOXs (CS@dsSzCOXs) established insecticidal effect adults via mixing wheat seeds, with mortality reaching 50% on day ten. Consequently, this nanoparticle-mediated expected be an effective approach control.
Language: Английский
Citations
0
Agriculture, Journal Year: 2025, Volume and Issue: 15(6), P. 642 - 642
Published: March 18, 2025
Concerns about the environmental and health risks of synthetic insecticides are driving search for alternative pest control methods. Leaf-cutting ants (LCAs), one most significant pests in neotropics, cause substantial economic damage to agriculture present challenges due their complex biology ecology. While chemical remains primary strategy, its intensive use has negative impacts, promotes resistance, endangers non-target species, including plants, animals, humans. This review describes LCAs, examines traditional methods suggests strategies such as entomopathogenic fungi (EPFs) combined with sublethal doses insecticides, plant essential oils (EOs), RNAi techniques. Here, we emphasize need address LCA management sustainably by investigating ecology at both “colony” “individual” levels. Colony-level factors include morphology, life cycle, behavior, division labor, nest structure, while individual-level mechanisms involve sensory, biochemical, behavioral adaptations garden sterilization decontamination. also highlights potential insecticide EPFs induce changes worker mortality, it details mode action EOs promising strategies. The integration biological approaches could offer sustainable alternatives insecticides.
Language: Английский
Citations
0
Current Opinion in Environmental Science & Health, Journal Year: 2025, Volume and Issue: unknown, P. 100612 - 100612
Published: March 1, 2025
Language: Английский
Citations
0
Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 205, P. 106114 - 106114
Published: Sept. 1, 2024
Language: Английский
Citations
2