Expression, characterization, and application of an aldo-keto reductase mined from Bacillus velezensis Vel-HNGD-F2 for deoxynivalenol biodegradation DOI
Junxia Liu, Peng Li, Lizhi Xiao

et al.

Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: unknown, P. 115159 - 115159

Published: Nov. 1, 2024

Language: Английский

Characterization of the Phosphotransferase from Bacillus subtilis 1101 That Is Responsible for the Biotransformation of Zearalenone DOI Creative Commons
Yuzhuo Wu, Qun Zhou, Junqiang Hu

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(1), P. 21 - 21

Published: Jan. 3, 2025

Bacillus microorganisms play an important role in the zearalenone (ZEA) biotransformation process natural environments. The phosphotransferase pathway is both widespread and relatively well conserved. However, reaction kinetics of these phosphotransferases remain poorly understood, their catalytic activities are suboptimal. In this study, a ZEA phosphotransferase, ZPH1101, was identified from subtilis 1101 using genome sequencing. product transformed by ZPH1101 as phosphorylated (ZEA-P) through LC-TOF-MS/MS analysis. experiments conducted on MCF-7 cells demonstrated that ZEA-P exhibited lower level estrogenic toxicity than ZEA. optimal conditions for were determined to be 45 °C pH 8.0. maximum velocity (Vmax), Michaelis constant (Km), (kcat) calculated fitting 16.40 μM·s-1·mg-1, 18.18 μM, 54.69 s-1, respectively. Furthermore, adding 1 mmol/L Fe2+ or Fe3+ system increased efficiency converting 100% relative containing solely ATP Mg2+, suggesting low concentrations can improve ZPH1101-mediated transformation This study contributes enzymatic removal broadens spectrum strain enzyme options available researchers detoxification efforts.

Language: Английский

Citations

1
Bacillus amyloliquefaciens HNGD-DF2 from Chinese Tofu: A potential thermophilic agent for AFB1 and ZEN degradation in wheat and maize DOI

Nazish Muzaffar,

Weibin Ma, Yuhui Yang

et al.

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111178 - 111178

Published: Jan. 1, 2025

Language: Английский

Citations

0
Designing flexible aliphatic linker based molecular imprinted covalent organic framework for rapid and selective extraction and detection of trace zearalenone DOI
Lihong Su, Hai‐Long Qian, Shuting Xu

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 490, P. 137778 - 137778

Published: Feb. 26, 2025

Language: Английский

Citations

0
Characterization, mechanism, and application of aldolase: a patulin-degrading enzyme from Kluyveromyces Marxianus YG-4 DOI

Mengge Ning,

Qi Guo, Peng Guo

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106345 - 106345

Published: March 1, 2025

Language: Английский

Citations

0
Biodegradation of ZEN by Bacillus mojavensis L-4: analysis of degradation conditions, products, degrading enzymes, and whole-genome sequencing, and its application in semi-solid-state fermentation of contaminated cornmeal DOI Creative Commons
Jong‐Soo Chang, Wenxuan Dong,

Shansong Gao

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Zearalenone (ZEN), a naturally occurring estrogenic mycotoxin prevalent in cereals and animal feed, poses significant challenge to livestock industry owing its detrimental effects on reproduction. In this study, the strains with high degradation rate were screened through co-culture ZEN, identified by bacterial morphology, 16S rDNA sequencing whole genome sequencing. The detoxification effect of L-4 strain ZEN was evaluated under different concentration, treatment time, pH value temperature, products identified, contaminated corn meal evaluated. degrading enzyme sequence obtained protein analysis L-4, verified molecular binding addition catalase. We isolated Bacillus mojavensis from cecal content laying hens, which demonstrated exceptional ZEN-degrading efficiency. Under optimized conditions (pH 7.0, 37 °C), completely degraded 0.5–1.0 μg/mL into less toxic 15-OH-ZEN within 24 h. Importantly, achieved 49.41% for cornmeal. Whole-genome revealed presence genes enzymes. particular, efeB 3668, peroxidase-like homology (95.91%) BsDyP subtilis , played key role primarily hydrogen bonding hydrophobic interactions. Thus, rapid effective B. coupled adaptability diverse environments, underscores potential application safeguarding health mitigating environmental pollution.

Language: Английский

Citations

0
Research diversity and advances in simultaneous removal of multi-mycotoxin DOI

Fred Mwabulili,

Peng Li,

Jinghao Shi

et al.

Toxicon, Journal Year: 2024, Volume and Issue: unknown, P. 108106 - 108106

Published: Sept. 1, 2024

Language: Английский

Citations

3
Expression, characterization, and application of an aldo-keto reductase mined from Bacillus velezensis Vel-HNGD-F2 for deoxynivalenol biodegradation DOI
Junxia Liu, Peng Li, Lizhi Xiao

et al.

Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: unknown, P. 115159 - 115159

Published: Nov. 1, 2024

Language: Английский

Citations

1