Synthesising human milk oligosaccharide using biotechnology-driven enzymes

Systems Microbiology and Biomanufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

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Multistrategy Optimization for High-Yield 3-Fucosyllactose Production in Escherichia coli BL21 Star (DE3)

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5385 - 5394

Published: Feb. 19, 2025

3-Fucosyllactose (3-FL), an essential component of human milk oligosaccharides, is crucial for infant health. However, the extraction 3-FL from presents a significant challenge. In this study, E. coli BL21 Star (DE3) was engineered biosynthesis, and gene combinations metabolic pathways were optimized to obtain high-yield production strain. First, module integrated into coli. Second, efficient alpha-1,3-fucosyltransferase screened. Then, different integration sites used optimize precursor synthesis cluster transferase genes screen transporter proteins glycerol utilization that could enhance production. The 3FL05-1 strain yielded titer 11.26 g/L in shake flasks. Further amplification 5 L bioreactor resulted 60.24 g/L, which represents highest reported date.

Language: Английский

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Multidimensional Engineering of Escherichia coli MG1655 for the Efficient Biosynthesis of Difucosyllactose

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5405 - 5413

Published: Feb. 24, 2025

Difucosyllactose (DFL), a representative bisfucosylated oligosaccharide found in human milk, has garnered significant attention due to its immense health benefits. To date, several plasmid-based engineered strains have been established for DFL synthesis. However, these face challenges such as antibiotic dependence and plasmid instability, which limit their commercial application the food industry. For this, plasmid-free Escherichia coli MG1655 were by integrating multicopy numbers of SAMT fut3Bc into genome overexpressing key genes involved GDP-l-fucose pathway. enhance catalytic efficiency, Fut3Bc was mutated based on AlphaFold 3, obtaining beneficial mutant (F24Y). The optimized strain, MGA2S-5, containing 2 copies 4 (F24Y) obtained. Eventually, strain MGA2S-5 synthesized 35.04 g/L 7 L bioreactor fed-batch cultivation, with no intermediate products remaining medium.

Language: Английский

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Structure, Function, Synthesis and Improved Strategies of Fucosylated Human Milk Oligosaccharides and Their Future Perspectives: A Review

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106584 - 106584

Published: April 1, 2025

Language: Английский

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