Rapid Construction of Liquid-like Surfaces via Single-Cycle Polymer Brush Grafting for Enhanced Antifouling in Microfluidic Systems DOI Creative Commons
Feng Wu, Jing Xu, Yuanyuan Liu

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(10), P. 1241 - 1241

Published: Oct. 9, 2024

Liquid-like surfaces have demonstrated immense potential in their ability to resist cell adhesion, a critical requirement for numerous applications across various domains. However, the conventional methodologies preparing liquid-like often entail complex multi-step polymer brush modification process, which is not only time-consuming but also presents significant challenges. In this work, we developed single-cycle strategy build by leveraging high-molecular-weight bis(3-aminopropyl)-terminated polydimethylsiloxane, significantly simplifies preparation process. The resultant surface endowed with exceptional slipperiness, effectively inhibiting bacterial colonization and diminishing adherence of platelets. Moreover, it offers promising implications reducing dependency on anticoagulants microfluidic systems constructed from PDMS, all while sustaining its antithrombotic attributes.

Language: Английский

Microencapsulation of Probiotics for Enhanced Stability and Health Benefits in Dairy Functional Foods: A Focus on Pasta Filata Cheese DOI Creative Commons
Vita D’Amico, Michele Cavaliere,

Marianna Ivone

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 185 - 185

Published: Feb. 2, 2025

Probiotics provide significant health benefits, but their viability is often compromised during production, storage, and passage through the gastrointestinal tract. These challenges hinder effective incorporation into functional applications, particularly in dairy foods, which factors such as acidity, oxygen exposure, storage conditions negatively impact cell survival. The focus was on pasta filata cheeses. Indeed, use of probiotics cheeses presents due to specific manufacturing processes, encompass application high temperatures other harsh conditions. can adversely affect availability probiotic microorganisms. However, microencapsulation has emerged a promising solution, offering protective barrier that enhances stability, improves survival rates, facilitates targeted release environment. This review examines pivotal role stabilising for emphasising its relevance high-value food systems. Functional including foods designed offer essential nutritional benefits promote host health, play crucial disease prevention immune system support, reducing risk infections physiological impairments. Key technologies are analysed, focusing limitations, related scalability industrial implementation. Additionally, this discusses strategies optimise formulations, ensure sensory quality final products, explore future opportunities expanding innovative applications align with growing consumer demand health-promoting solutions.

Language: Английский

Citations

2
Dietary probiotic-derived extracellular vesicles as delivery systems of bioactive compounds to maintain intestinal homeostasis DOI
Kuiyou Wang, Xue–Qian Li, Kexin Huang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159546 - 159546

Published: Jan. 1, 2025

Language: Английский

Citations

1
Advances in the preparation, applications, challenges, and future trends of polysaccharide‐based gels as food‐grade delivery systems for probiotics: A review DOI
Jianwei Zang, Zhenliang Yin,

Huidan Ouyang

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 1, 2025

Abstract Probiotics are highly regarded for their multiple functions, such as regulating gut health, enhancing the immune system, and preventing chronic diseases. However, stability in harsh environments targeted release remain significant challenges. Therefore, exploring effective protection delivery strategies to ensure of probiotics is critically important. Polysaccharides, known non‐toxicity, excellent biocompatibility, superior biodegradability, show broad prospects probiotic by forming physical barriers protect probiotics. Particularly, polysaccharide‐based gels (PBGs), with unique “spider‐web” like structure, capture probiotics, significantly efficacy. This review discusses common polysaccharides used PBG preparation, classification synthesis food applications, advantages PBGs systems. Despite potential, challenges inconsistent gel properties need improved remain. Future research should focus on developing novel materials higher biodegradability mechanical strength, optimizing physicochemical cross‐linking methods, well designing multilayered structures more precise control. Additionally, co‐delivery prebiotics, active ingredients, or multi‐strain systems could further enhance efficacy delivery.

Language: Английский

Citations

0
Microfluidic design and preparation of hydrogel microcapsules of Mesona chinensis polysaccharide: Characterization, pH-responsive behavior and gastrointestinal protection for Lactobacillus plantarum DOI

Pengkun Lei,

Lin Luo, Peng Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140446 - 140446

Published: Jan. 1, 2025

Language: Английский

Citations

0
Overview of Glycometabolism of Lactic Acid Bacteria During Freeze-Drying: Changes, Influencing Factors, and Application Strategies DOI Creative Commons

Tchouli Noufeu,

Yueqin Li,

Ndeye Fatou Toure

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 743 - 743

Published: Feb. 22, 2025

Lactic acid bacteria (LAB) play a vital role in food fermentation and probiotics microeconomics. Freeze-drying (FD) is commonly used method for preserving LAB powder to extend its shelf life. However, FD induces thermal, osmotic, mechanical stresses that can impact the glycometabolism of LAB, which process converting carbohydrates into energy. This review explores effect on glycometabolism, factors influencing feasible strategies LAB. During three stages FD, freezing, primary drying or sublimation, second drying, glycolytic activity disrupted freezing stage; further, function enzymes such as hexokinase, phosphofructokinase, pyruvate kinase hindered, adenosine triphosphate (ATP) production drops significantly sublimation these enzyme activities ATP nearly cease exopolysaccharide (EPS) synthesis alters during secondary stage. Factors strain variations, pretreatment techniques, growth medium components, parameters, water influence changes. To counteract effects like cryoprotectants, encapsulation, genetic engineering help preserve their glycometabolic activity. These methods protect from harsh conditions, safeguarding flux enzymatic processes involved carbohydrate metabolism. A deeper understanding changes essential optimizing enhancing use food, medicine, biotechnology, ultimately improving performance upon rehydration.

Language: Английский

Citations

0
Live Probiotics-Loaded Delivery Systems for the Treatment of Infected Wounds DOI
Oksana Gerulis, Karin Kogermann

NATO science for peace and security series. A, Chemistry and biology, Journal Year: 2025, Volume and Issue: unknown, P. 51 - 78

Published: Jan. 1, 2025

Language: Английский

Citations

0
Transient Implantable Electronics for Postsurgery Preventive Medicine DOI Creative Commons
Hadi Mirzajani, Parviz Zolfaghari, Sattar Akbari Nakhjavani

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract The field of postoperative care has seen a remarkable shift toward the utilization electronic implantable devices, including sensors, biosensors, stimulators, and drug delivery systems, all designed with biodegradable form factor wireless data/power transmission capability. These technologies hold immense potential for postsurgery out‐of‐hospital monitoring during postdischarged period, where continuous physiological signals is lacking. Furthermore, these devices eliminate need secondary surgeries required device retrieval as they can safely degraded in body, thus enhancing patient recovery. This review delves into latest advancements examining their application vital signs, innovative communication powering employ, materials that enable function. analysis extends to evaluating efficacy limitations across various medical applications. Moreover, it explores future research directions, emphasizing material advancements, miniaturization, customization, integration artificial intelligence create closed‐loop therapeutic systems. comprehensive underscores transformative outlines pathway innovations this dynamic field.

Language: Английский

Citations

3
Rewiring Tryptophan Metabolism via Programmable Probiotic Integrated by Dual-Layered Microcapsule Protects against Inflammatory Bowel Disease in Mice DOI
Wen Li, Yichen Liu, Xiaoming Zheng

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(52), P. 35443 - 35464

Published: Nov. 28, 2024

Intestinal dysbiosis and the associated l-tryptophan metabolic disorder are pivotal in inflammatory bowel disease progression, leading to a compromised intestinal barrier integrity. Remedying dysfunction tryptophan metabolism has emerged as promising therapeutic strategy. Herein, we reprogram situ by EcN-TRP@A/G, encapsulating engineered probiotic, EcN-TRP, with enhanced synthesis capacity, for sustained modulation, thereby restoring function microbial homeostasis. The pH-responsive dual-layered EcN-TRP@A/G microcapsule developed via high-voltage electrospraying liquid interface self-assembly, preserved probiotic viability harsh gastrointestinal milieu, facilitated targeted colon release. Bioluminescent tracking mice reveals 22.84-fold increase distribution compared naked EcN-TRP. Targeted metabolomics highlights EcN-TRP@A/G's modulation of tryptophan-indole pathway. Oral administration elevates indole metabolites, particularly indole-3-acetic acid indole-3-propionic acid, tissue up 7 days. In IBD mice, improves permeability, reduces inflammation, recovers gut microbiome enhancing beneficial bacteria abundance like Prevotellaceae_UCG-001 Anaerostipes while suppressing pathogenic strains Escherichia-Shigella. Our findings offer cost-effective approach, harnessing potential through modifications effective treatment.

Language: Английский

Citations

2
Rapid Construction of Liquid-like Surfaces via Single-Cycle Polymer Brush Grafting for Enhanced Antifouling in Microfluidic Systems DOI Creative Commons
Feng Wu, Jing Xu, Yuanyuan Liu

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(10), P. 1241 - 1241

Published: Oct. 9, 2024

Liquid-like surfaces have demonstrated immense potential in their ability to resist cell adhesion, a critical requirement for numerous applications across various domains. However, the conventional methodologies preparing liquid-like often entail complex multi-step polymer brush modification process, which is not only time-consuming but also presents significant challenges. In this work, we developed single-cycle strategy build by leveraging high-molecular-weight bis(3-aminopropyl)-terminated polydimethylsiloxane, significantly simplifies preparation process. The resultant surface endowed with exceptional slipperiness, effectively inhibiting bacterial colonization and diminishing adherence of platelets. Moreover, it offers promising implications reducing dependency on anticoagulants microfluidic systems constructed from PDMS, all while sustaining its antithrombotic attributes.

Language: Английский

Citations

0