Transcriptomic Regulatory Mechanisms of Isoflavone Biosynthesis in Trifolium pratense

Agronomy, Journal Year: 2025, Volume and Issue: 15(5), P. 1061 - 1061

Published: April 27, 2025

Isoflavones are important secondary metabolites in leguminous plants with significant physiological functions and economic value. However, the genetic variation, transcriptional regulation, metabolic pathways governing isoflavone biosynthesis Trifolium pratense remain largely unexplored. In this study, we systematically analyzed 500 accessions of T. for content performed RNA-seq-based transcriptomic profiling to investigate molecular mechanisms underlying biosynthesis. Cluster analysis revealed distinct profiles between high- (H1, H2, H3) low-isoflavone (L1, L2, L3) groups. GO KEGG pathway enrichment analyses identified key pathways, including phenylpropanoid metabolism, flavonoid biosynthesis, carbohydrate hormone signaling, which play crucial roles regulation. Weighted gene co-expression network (WGCNA) three modules—MEblue, MEturquoise, MEyellow—strongly correlated content. The MEturquoise MEyellow modules were upregulated high-isoflavone groups enriched lipid suggesting that these actively promote accumulation. Conversely, MEblue module, highly expressed groups, was sugar metabolism MAPK indicating a potential flux shift away from metabolism. Moreover, rate-limiting enzymes (PAL, C4H, 4CL, CHS, IFS) exhibited higher expression highlighting their importance precursor supply enzymatic catalysis. Additionally, transcription factors such as MYB, WRKY, NAC regulators complex interplay hormonal, circadian, environmental signals. This study provides comprehensive framework understanding identifies regulatory genes could be targeted improvement, engineering, breeding. findings offer valuable insights into enhancing production legumes agricultural, nutritional, pharmaceutical applications.

Language: Английский

Dissection of genetic architecture for desirable traits in sugarcane by integrated transcriptomics and metabolomics

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 280, P. 136009 - 136009

Published: Sept. 25, 2024

Language: Английский