Molecular surveillance of resistance mutations in invasive populations of Spodoptera frugiperda in Europe, for evidence‐based pest control DOI Creative Commons
Konstantinos Mavridis, Vasiliki Evangelοu,

Alexandra M Grigoriadou

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract BACKGROUND The invasive fall armyworm ( Spodoptera frugiperda , FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence‐based control strategies. In this study, we analyzed target‐site insecticide mutations in FAW populations from Greece inform addition, DNA barcoding through cytochrome oxidase subunit 1 COI ) gene sequencing was used trace the pest's geographic origin and potential invasion pathways. RESULTS All specimens were identified as rice strain, exhibiting two almost balanced haplotypes (Haplotype 1: 58.6%; Haplotype 2: 41.4%), suggesting likely single, genetically diverse source population. Resistance‐associated ABCC2 (A > G single‐nucleotide polymorphism (SNP); up 80.9%) Ace‐1 (F290V: 37.5%; A201S: 3.85%), conferring Bacillus thuringiensis (Bt) organophosphates/carbamates, respectively. By contrast, no resistance‐associated detected for other key insecticides (diamides, pyrethroids, oxadiazines, spinosyns, avermectins), their current efficacy Greece. CONCLUSION This study provides critical baseline monitoring Europe, supporting development of sustainable integrated strategies line with European Union Green Deal. Continuous molecular diagnostics, alongside complementary bioassays, recommended mitigate impact on agriculture. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Monitoring insecticide resistance and target-site mutations in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) in China DOI
Baojuan Zeng, Jian Ding,

Yajuan Xiao

et al.

Journal of Economic Entomology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

The fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), a notorious invasive pest, has been widely monitored for insecticide resistance. Since its invasion of China in late 2018, early to mid-stage monitoring resistance is particularly crucial inform effective control strategies. Resistance ratios (RRs) derived from bioassays offer valuable insights into the overall levels field populations, while detection allele frequencies helps uncover potential causes variation. In this study, we established baseline susceptibility third-instar larvae 7 insecticides using laboratory strain Xinzheng2019 and assessed 9 populations collected central southern between 2022 2023. Compared susceptible strain, 2 showed low-level (RR = 5-10) indoxacarb, one exhibited chlorantraniliprole. Minor 3-5) was observed lambda-cyhalothrin, 5 All remained < 3) spinetoram, emamectin benzoate, chlorfenapyr, lufenuron. Molecular analysis 11 mutation sites across 3 key resistance-related genes (Ace-1, GluCl, voltage-gated sodium channel [VGSC]) revealed that 52.1% all tested samples carried either homozygous or heterozygous alleles at F290V locus Ace-1 gene, no mutations were detected other sites. Our findings status S. provide guidance chemical use.

Language: Английский

Citations

0
The miRNA-275 Targeting RpABCG23L is Involved in Pyrethroid Resistance in the Bird Cherry-Oat Aphid, a Serious Agricultural Pest DOI

Hongcheng Tang,

Chao Chen, Sisi Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Rhopalosiphum padi is a global agricultural pest which had developed resistance to different insecticides. The ATP-binding cassette (ABC) transporter plays an important role in insecticide resistance. However, ABC transporters' and regulatory mechanism mediating R. 's response pyrethroids are unclear. In this study, we found that RpABCG23L was significantly overexpressed the pyrethroid-resistant strains of padi. Knockdown increased susceptibility lambda-cyhalothrin bifenthrin. Luciferase reporter gene analysis showed miR-275 binds coding region down-regulates its expression. Injection mimics reduced expression bifenthrin, while inhibitor injection enhanced tolerance both results provide theoretical basis for understanding miRNA-mediated pyrethroid resistance, open up new way development miRNA-based biopesticides.

Language: Английский

Citations

0
Insight into the Underlying Molecular Toxic Mechanisms of Cyantraniliprole and Broflanilide against Different Targets with Glutathione Transferase Phi8 from Arabidopsis thaliana DOI
Fan Yang, Yifan Chen, Xiaomin Hou

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(11), P. 6537 - 6544

Published: March 5, 2025

As two typical kinds of diamide insecticides, cyantraniliprole and broflanilide are characterized by diverse target receptors completely distinctive regulation mechanisms. However, the systematical evaluation oxidative damage on plants induced still remains elusive. In this study, toxic effects were investigated in biochemical physiological aspects using AtGSTF8 as a biomarker. First, not only brought about much more detrimental impacts growth status plant seedlings but also resulted significant upregulation gene expression compared to broflanilide. Then, glutathione S-transferase activities decreased greatly with treatment these insecticides. Moreover, biolayer interferometry analysis confirmed interactions both AtGSTF8, especially for stronger binding affinity, which conformed molecular docking results. At last, even adverse structural conformations upon observed.

Language: Английский

Citations

0
Molecular surveillance of resistance mutations in invasive populations of Spodoptera frugiperda in Europe, for evidence‐based pest control DOI Creative Commons
Konstantinos Mavridis, Vasiliki Evangelοu,

Alexandra M Grigoriadou

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract BACKGROUND The invasive fall armyworm ( Spodoptera frugiperda , FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence‐based control strategies. In this study, we analyzed target‐site insecticide mutations in FAW populations from Greece inform addition, DNA barcoding through cytochrome oxidase subunit 1 COI ) gene sequencing was used trace the pest's geographic origin and potential invasion pathways. RESULTS All specimens were identified as rice strain, exhibiting two almost balanced haplotypes (Haplotype 1: 58.6%; Haplotype 2: 41.4%), suggesting likely single, genetically diverse source population. Resistance‐associated ABCC2 (A > G single‐nucleotide polymorphism (SNP); up 80.9%) Ace‐1 (F290V: 37.5%; A201S: 3.85%), conferring Bacillus thuringiensis (Bt) organophosphates/carbamates, respectively. By contrast, no resistance‐associated detected for other key insecticides (diamides, pyrethroids, oxadiazines, spinosyns, avermectins), their current efficacy Greece. CONCLUSION This study provides critical baseline monitoring Europe, supporting development of sustainable integrated strategies line with European Union Green Deal. Continuous molecular diagnostics, alongside complementary bioassays, recommended mitigate impact on agriculture. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Citations

0