Combinatorial Targeting of Common Docking and ATP Binding Sites on Mps1 MAPK for Management of Pathogenic Fungi DOI
Zhiwei Kong,

Saijie Li,

Jianbin Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Resistance in pathogenic fungi necessitates the development of fungicides with new mechanisms action. The Mps1 MAPK Magnaporthe oryzae, pathogen rice blast disease, has been shown to be a molecular target for fungicide research. Here, we present compound TAK-733 that interacts common docking (CD) site and can used combination ATP-competitive inhibitors. We initially identified compounds PLX-4720 interact Mps1. Subsequent assays show is an inhibitor, whereas binds CD Mps1─an interaction its MAPKK─but not ATP-binding pocket as it does kinase MEK1. In vivo demonstrated exhibits combinational effects inhibitors A378–0. Collectively, having mechanism action suitable application management fungi.

Language: Английский

Melatonin—Angel of Plant Growth Regulation and Protection DOI Creative Commons

Huanyu Cai,

Jie Li, Jun Li

et al.

Advanced Agrochem, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

3
Diverse abietane diterpenoids as multifunctional antimicrobial effects from Salvia castanea DOI
Panpan Wang, Song Gao,

Shu‐Xia Liu

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Abstract BACKGROUND Phytopathogenic fungal diseases significantly impact the quality of vegetables and fruits in agriculture. Salvia castanea , a Tibetan medicinal plant, possessed anti‐inflammatory, antitumor, antimicrobial activities. Therefore, we investigated ingredients from this plant clarified their protective curative plants detail. RESULTS Bio‐guided isolation was conducted to obtain 23 abietane diterpenoids, including three new ones (Castanoids A–C). Most compounds exhibited significant antifungal activity against four fungi. Castanoid C ( 7 ) showed remarkable inhibition Sclerotinia sclerotiorum Valsa mali Botrytis cinerea with median effective concentration (EC 50 values 1.30, 0.84, 2.40 μg/mL, respectively, outperforming commercial agent trifloxystrobin. In vivo compound demonstrated 100% 82.26 ± 1.07% efficiency on rape leaves at 200 μg/mL. At 300 μg/ mL, it effectively controlled tomato rot 92.65 2.55% activity. The observation by transmission electron microscopy disclosed that inhibited growth fungi destroying cell membrane permeability caused leakage cellular components. Additionally, 11 exerted potent inhibitory effects Staphylococcus aureus Pseudomonas syringae pv. actinidae (Psa) minimum (MIC) value 6.25 μ m surpassing gentamicin. Its mechanism Psa may also involve damage. CONCLUSION result highlights antibacterial potential diterpenoids particularly C. These findings suggest broad applicability agriculture, especially for controlling rape, apple, crops. This research provides foundation developing natural, agents, advancing field disease management. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Combinatorial Targeting of Common Docking and ATP Binding Sites on Mps1 MAPK for Management of Pathogenic Fungi DOI
Zhiwei Kong,

Saijie Li,

Jianbin Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Resistance in pathogenic fungi necessitates the development of fungicides with new mechanisms action. The Mps1 MAPK Magnaporthe oryzae, pathogen rice blast disease, has been shown to be a molecular target for fungicide research. Here, we present compound TAK-733 that interacts common docking (CD) site and can used combination ATP-competitive inhibitors. We initially identified compounds PLX-4720 interact Mps1. Subsequent assays show is an inhibitor, whereas binds CD Mps1─an interaction its MAPKK─but not ATP-binding pocket as it does kinase MEK1. In vivo demonstrated exhibits combinational effects inhibitors A378–0. Collectively, having mechanism action suitable application management fungi.

Language: Английский

Citations

0