High-Level De Novo Production of (2S)-Naringenin in Yarrowia lipolytica Using Metabolic and Enzyme Engineering
Ziyun Ru,
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Mengsu Liu,
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Qihang Chen
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et al.
ACS Agricultural Science & Technology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 12, 2025
Language: Английский
Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica
Jun Xiang,
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Mengsu Liu,
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Xinglong Wang
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et al.
Synthetic and Systems Biotechnology,
Journal Year:
2025,
Volume and Issue:
10(3), P. 719 - 727
Published: Feb. 20, 2025
δ-Tocotrienol,
an
isomer
of
vitamin
E
with
anti-inflammatory,
neuroprotective
and
anti-coronary
arteriosclerosis
properties,
is
widely
used
in
health
care,
medicine
other
fields.
Microbial
synthesis
δ-tocotrienol
offers
significant
advantages
over
plant
extraction
chemical
methods,
including
increased
efficiency,
cost-effectiveness
environmental
sustainability.
However,
limited
precursor
availability
low
catalytic
efficiency
key
enzymes
remain
major
bottlenecks
the
biosynthesis
δ-tocotrienol.
In
this
study,
we
assembled
complete
biosynthetic
pathway
Yarrowia
lipolytica
enhanced
supply,
resulting
a
titre
102.8
mg/L.
The
rate-limiting
steps
was
then
through
various
strategies,
fusion
expression
homogentisate
phytyltransferaseand
tocopherol
cyclase,
semi-rational
design
SyHPT
multi-copy
integration
genes.
final
5
L
bioreactor
466.8
mg/L
following
fed-batchfermentation.
This
study
represents
first
successful
de
novo
Y.
lipolytica,
providing
valuable
insights
into
E-related
compounds.
Language: Английский
The activity annotation of peach glycosyltransferase PpUGT78B based on engineering bacterial anthocyanin biosynthesis
Ruyu Jing,
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Pengyu Wu,
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Pengfei Wang
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et al.
Plant Physiology and Biochemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 109913 - 109913
Published: April 1, 2025
Language: Английский
Increased thermal stability and catalytic efficiency of 3-ketosteroid Δ1-dehydrogenase5 from Arthrobacter simplex significantly reduces enzyme dosage in prednisone acetate biosynthesis
Rong Zhang,
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X. G. Luo,
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Danni Li
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et al.
International Journal of Biological Macromolecules,
Journal Year:
2024,
Volume and Issue:
unknown, P. 137855 - 137855
Published: Nov. 1, 2024
Language: Английский