Manganese Removal from the Seo-Gok Reservoir Water Using DNA Aptamers DOI Open Access

Daehyuk Jang,

Sun Young Lee, W Kim

et al.

Environments, Journal Year: 2025, Volume and Issue: 12(4), P. 99 - 99

Published: March 25, 2025

Manganese (Mn) is widely used in many industries but also biologically harmful when abundant the environment. While there are several commercially available methods for manganese removal from water, efficient and cost-effective solutions addressing contamination diverse environmental matrices remain limited. In this study, we developed a new method removing Mn contaminated lakes using an aptamer. The Seo-Gok Reservoir was selected as study area due to its significant levels of contamination. We first screened aptamers that bind through systematic evolution ligands by exponential enrichment (SELEX). Among 6 (from FA-M1 FA-M6), aptamer exhibited highest binding affinity with lowest Kd value 4.56 × 10−9 M. Potential Mn-binding sites were predicted analyzing secondary structures. To confirm proposed region, evaluated sequence homology aptamers. Aptamer specificity against metals. demonstrated could remove more than 95% aqueous sample; 99.9% then be recovered. removed 90% sample Mn-contaminated Reservoir, indicating can utilized ions

Language: Английский

Manganese Removal from the Seo-Gok Reservoir Water Using DNA Aptamers DOI Open Access

Daehyuk Jang,

Sun Young Lee, W Kim

et al.

Environments, Journal Year: 2025, Volume and Issue: 12(4), P. 99 - 99

Published: March 25, 2025

Manganese (Mn) is widely used in many industries but also biologically harmful when abundant the environment. While there are several commercially available methods for manganese removal from water, efficient and cost-effective solutions addressing contamination diverse environmental matrices remain limited. In this study, we developed a new method removing Mn contaminated lakes using an aptamer. The Seo-Gok Reservoir was selected as study area due to its significant levels of contamination. We first screened aptamers that bind through systematic evolution ligands by exponential enrichment (SELEX). Among 6 (from FA-M1 FA-M6), aptamer exhibited highest binding affinity with lowest Kd value 4.56 × 10−9 M. Potential Mn-binding sites were predicted analyzing secondary structures. To confirm proposed region, evaluated sequence homology aptamers. Aptamer specificity against metals. demonstrated could remove more than 95% aqueous sample; 99.9% then be recovered. removed 90% sample Mn-contaminated Reservoir, indicating can utilized ions

Language: Английский

Citations

0