Unveiling the N-Glycomic Diversity of Goat Lactoferrin during Lactation DOI
Yuyang Zhang,

Tianjiao Han,

Lanwei Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Goat lactoferrin is an N-glycoprotein, and its glycan moieties are essential for biological activity. However, the structures of these glycans, particularly sialylated isomers with α-2,3- or α-2,6-linkages, remain poorly characterized. Utilizing online hydrophilic liquid chromatography-tandem mass spectrometry, our study characterized N-glycans in goat across different lactation stages, putative 86 were presented, including 53 previously undetected ones. The content variety decreased from colostrum to mature milk, transitional milk exhibiting highest levels neutral high-mannose N-glycans. Colostrum was rich fucose- N-glycans, especially those α-2,6-linkages. Notably, α-2,6-linked N-glycan H5N4F1A1-1 7.09-fold 12.85-fold more abundant compared respectively. These findings provide valuable insights into structure could facilitate development functional products.

Language: Английский

Goat milk oligosaccharides: regulating infant immunity by intervention in the gut microbiota DOI
Sibo Liu, Xiaoling Zeng, Jing Li

et al.

Food & Function, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The health status of the growing infant is closely related to development gut microbiota during infancy, which also a major stimulator immune system.

Language: Английский

Citations

0
Unveiling the N-Glycomic Diversity of Goat Lactoferrin during Lactation DOI
Yuyang Zhang,

Tianjiao Han,

Lanwei Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Goat lactoferrin is an N-glycoprotein, and its glycan moieties are essential for biological activity. However, the structures of these glycans, particularly sialylated isomers with α-2,3- or α-2,6-linkages, remain poorly characterized. Utilizing online hydrophilic liquid chromatography-tandem mass spectrometry, our study characterized N-glycans in goat across different lactation stages, putative 86 were presented, including 53 previously undetected ones. The content variety decreased from colostrum to mature milk, transitional milk exhibiting highest levels neutral high-mannose N-glycans. Colostrum was rich fucose- N-glycans, especially those α-2,6-linkages. Notably, α-2,6-linked N-glycan H5N4F1A1-1 7.09-fold 12.85-fold more abundant compared respectively. These findings provide valuable insights into structure could facilitate development functional products.

Language: Английский

Citations

0