Two carboxyl/choline esterase (CCE) genes, TuCCE11 and TuCCE34, are related to abamectin resistance in Tetranychus urticae Koch

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143287 - 143287

Published: April 17, 2025

Language: Английский

Nanocarrier-mediated RNAi of CYP9E2 and CYB5R enhance susceptibility of invasive tomato pest, Tuta absoluta to cyantraniliprole

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

Cyantraniliprole, a second-generation anthranilic diamide, is widely used to manage lepidopteran pests, including the invasive tomato pinworm Tuta absoluta (Meyrick). However, cyantraniliprole’s resistance mechanisms and associated fitness costs in T. remain underexplored. Here, we investigated of cyantraniliprole-resistant strain (CyanRS) via nanocarrier-mediated RNA interference (RNAi). Results showed that egg incubation period developmental durations larval pupal stages were significantly prolonged CyanRS population compared susceptible (SS). Further, adult emergence, longevities male female, fecundity, hatching rate reduced individuals. The mRNA expression levels cytochrome b5 reductase ( CYB5R ) P450 CYP9E2 analyzed using RT-qPCR explore their potential involvement cyantraniliprole . Phylogenetic motif analysis indicated evolutionary functional conservation with other insect species, especially Lepidopterans. Notably, nanocarrier-encapsulated dsRNA targeting genes levels. activity was substantially decreased after knockdown CYP92 genes. This increased susceptibility resistant cyantraniliprole, leading higher mortality than controls. These findings show might play key role evolution current study provides in-depth insights understand underlying this herbivore.

Language: Английский

Status and mechanism of insecticide resistance of Ceratitis capitata (Diptera: Tephritidae) populations in East Mediterranean Region in Türkiye

Phytoparasitica, Journal Year: 2025, Volume and Issue: 53(3)

Published: April 26, 2025

Language: Английский