Collagen Peptides and Saccharomyces boulardiiCNCM I‐745 Attenuate Acetic Acid‐Induced Colitis in Rats by Modulating Inflammation and Barrier Permeability DOI Creative Commons
Öykü Altınok, Murat Baş, Elif Gelenli Dolanbay

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: April 1, 2025

ABSTRACT Ulcerative colitis (UC) is an inflammatory bowel disease characterized by recurrent episodes of inflammation and tissue damage, with limited treatment options. This study aimed to investigate the effects collagen peptides Saccharomyces boulardii on acetic acid (AA)‐induced colitis. Thirty‐six male Sprague–Dawley rats were randomly divided into following four groups: normal control (NC), (CC), peptide (CP; 0.6 g/kg/day), S. (SB; 250 mg/day). Colitis was induced intrarectal administration AA in all groups except NC, treatments administered daily for 7 days. The therapeutic evaluated assessing activity index (DAI), colon mass index, macroscopic microscopic histopathological changes, zonula occludens (ZO)‐1 protein expression, myeloperoxidase (MPO) activity. results showed that CP SB substantially alleviated DAI scores ( p < 0.05) reduced index. Colon damages improved compared CC group 0.01). Histologically, both cell infiltration, crypt ulceration, showing a slightly more pronounced effect. Immunohistochemical analysis revealed significant restoration ZO‐1 expression treated groups, indicating improvement intestinal barrier integrity Furthermore, MPO significantly These findings are consistent previous studies highlight anti‐inflammatory barrier‐enhancing probiotics UC models.

Language: Английский

Iturin A Potentiates Differentiation of Intestinal Epithelial Defense Cells by Modulating Keap1/Nrf2 Signaling to Mitigate Oxidative Damage Induced by Heat-Stable Enterotoxin B DOI Creative Commons

Geng-xiu Zan,

Hong Qu,

Xinyang Li

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 478 - 478

Published: April 16, 2025

Intestinal stem cells (ISCs) maintain epithelial renewal through their proliferation and differentiation capabilities, responding to various intestinal insults. However, the impact of iturin A, a natural antimicrobial peptide, on ISC viability its potential mitigate heat-stable enterotoxin b (STb)-induced damage remains unclear. Our recent study demonstrated that oral administration A enhances tight junction protein expression, accelerates crypt-villus regeneration, restores barrier integrity in STb-exposed mice. Furthermore, promotes differentiation, significantly increasing numbers goblet Paneth jejunum following STb exposure. Notably, regulates homeostasis by scavenging reactive oxygen species (ROS), while elevating total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) levels both serum jejunal mucosa. Mechanistically, facilitates nuclear factor-erythroid 2- related factor 2 (Nrf2) release disrupting Kelch-like ECH-associated 1 (Keap1), leading upregulation enzyme 4 (GPX4). In conclusion, our findings indicate alleviates oxidative stress induced modulation Keap1/Nrf2 pathway into cells, thereby enhancing resistance STb-induced damage.

Language: Английский

Citations

0
Collagen Peptides and Saccharomyces boulardiiCNCM I‐745 Attenuate Acetic Acid‐Induced Colitis in Rats by Modulating Inflammation and Barrier Permeability DOI Creative Commons
Öykü Altınok, Murat Baş, Elif Gelenli Dolanbay

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: April 1, 2025

ABSTRACT Ulcerative colitis (UC) is an inflammatory bowel disease characterized by recurrent episodes of inflammation and tissue damage, with limited treatment options. This study aimed to investigate the effects collagen peptides Saccharomyces boulardii on acetic acid (AA)‐induced colitis. Thirty‐six male Sprague–Dawley rats were randomly divided into following four groups: normal control (NC), (CC), peptide (CP; 0.6 g/kg/day), S. (SB; 250 mg/day). Colitis was induced intrarectal administration AA in all groups except NC, treatments administered daily for 7 days. The therapeutic evaluated assessing activity index (DAI), colon mass index, macroscopic microscopic histopathological changes, zonula occludens (ZO)‐1 protein expression, myeloperoxidase (MPO) activity. results showed that CP SB substantially alleviated DAI scores ( p < 0.05) reduced index. Colon damages improved compared CC group 0.01). Histologically, both cell infiltration, crypt ulceration, showing a slightly more pronounced effect. Immunohistochemical analysis revealed significant restoration ZO‐1 expression treated groups, indicating improvement intestinal barrier integrity Furthermore, MPO significantly These findings are consistent previous studies highlight anti‐inflammatory barrier‐enhancing probiotics UC models.

Language: Английский

Citations

0