Functional Analysis of Two Carboxylesterase Genes Involved in Beta-Cypermethrin and Phoxim Resistance in Plutella xylostella (L.) DOI Creative Commons
Ran Li, Liang Liang, Yaohua Zhao

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 2781 - 2781

Published: Nov. 23, 2024

Enhanced expression of carboxylesterase (CarE) genes is an important mechanism insecticide resistance in pests. However, their roles multi-insecticide have rarely been reported. Herein, two CarE (PxαE6 and PxαE9) were identified; relative levels three multi-insecticide-resistant populations P. xylostella (HN, GD-2017 GD-2019) 2.69- to 15.32-fold higher than those the sensitive population, they considerably overexpressed at larval stage midgut 4th instar. PxαE6 PxαE9 knockdown increased susceptibility GD-2019 larvae phoxim or/and beta-cypermethrin. The recombinant expressed Escherichia coli exhibited high hydrolysis activity towards α-NA. GC–MS LC–MS/MS assays revealed that could metabolize beta-cypermethrin with efficiency determinations 51.6% 21.1%, respectively, while 12.0%. Homology modelling, molecular docking molecular-dynamics simulation analyses demonstrated fit well into active pocket stably bind or PxαE9. These results show overexpression involved populations, a finding which sheds light on mechanisms insect

Language: Английский

Functional Analysis of Two Carboxylesterase Genes Involved in Beta-Cypermethrin and Phoxim Resistance in Plutella xylostella (L.) DOI Creative Commons
Ran Li, Liang Liang, Yaohua Zhao

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 2781 - 2781

Published: Nov. 23, 2024

Enhanced expression of carboxylesterase (CarE) genes is an important mechanism insecticide resistance in pests. However, their roles multi-insecticide have rarely been reported. Herein, two CarE (PxαE6 and PxαE9) were identified; relative levels three multi-insecticide-resistant populations P. xylostella (HN, GD-2017 GD-2019) 2.69- to 15.32-fold higher than those the sensitive population, they considerably overexpressed at larval stage midgut 4th instar. PxαE6 PxαE9 knockdown increased susceptibility GD-2019 larvae phoxim or/and beta-cypermethrin. The recombinant expressed Escherichia coli exhibited high hydrolysis activity towards α-NA. GC–MS LC–MS/MS assays revealed that could metabolize beta-cypermethrin with efficiency determinations 51.6% 21.1%, respectively, while 12.0%. Homology modelling, molecular docking molecular-dynamics simulation analyses demonstrated fit well into active pocket stably bind or PxαE9. These results show overexpression involved populations, a finding which sheds light on mechanisms insect

Language: Английский

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