
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 17, 2025
Meliaceous plants such as Azadirachta indica (neem) and Melia azedarach (chinaberry) contain large amounts of limonoids with unique anti-insect activities. However, genes responsible for downstream modifications are not well known. Here, we improve the genome assemblies neem chinaberry to telomere-to-telomere (T2T) level. Allopatric speciation two is confirmed by lineage-specific inversion chromosome 12 in lineage. We further identify BAHD-acetyltransferases (ATs) (MaAT8824 MaAT1704) that catalyse acetylation at both C-12 C-3 hydroxyl groups limonoids, whereas syntenic copy (AiAT0635) does possess this activity. A critical N-terminal region (SAGAVP) crucial AiAT0635, swapping it into MaAT8824 version (CHRSSG) can endow Our improved provide insights allopatric neem, limonoid biosynthesis chemical diversity meliaceous plants. Biosynthetic pathway after azadirone still unclear. authors report genomes tree reveal acetyltransferases determine structure species.
Language: Английский