Preparation of Sphingan Oligosaccharides by SpnR Hydrolysis and Their Prebiotic Effects on Intestinal Flora DOI
Jinsong Huang,

Qiao Qin,

Kecheng Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Oligosaccharides have the potential to act as prebiotics and can be obtained by enzymatic hydrolysis of polysaccharides. Sphingans are exopolysaccharides, including gellan, welan, diutan, which synthesized genus Sphingomonas. Sphingan lyase (SpnR) has attracted a great deal interest due its ability produce oligosaccharides through degradation sphingans. However, there few reports on catalytic mechanism hydrolysate analysis SpnR. Here, SpnR from Sphingomonas sp. HT-1 was heterologously expressed in Escherichia coli. The optimal temperature pH purified were 35 °C 7.2, respectively. good alkali resistance relatively mild optimum temperature. Na+ Fe3+ increased activities, contrast Zn2+ EDTA. could degrade sphingans, most suitable substrate welan. Analysis cleavage pattern revealed that acts specifically β-1,4-glycosidic bond obtain minimum trisaccharide sphingan (SpnOS). gut microbiota vitro showed SpnOS caused substantial modification intestinal microbiota, characterized an augmented proliferation beneficial bacterial populations, notably Bacteroides Lactobacillus.

Language: Английский

Preparation of Sphingan Oligosaccharides by SpnR Hydrolysis and Their Prebiotic Effects on Intestinal Flora DOI
Jinsong Huang,

Qiao Qin,

Kecheng Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Oligosaccharides have the potential to act as prebiotics and can be obtained by enzymatic hydrolysis of polysaccharides. Sphingans are exopolysaccharides, including gellan, welan, diutan, which synthesized genus Sphingomonas. Sphingan lyase (SpnR) has attracted a great deal interest due its ability produce oligosaccharides through degradation sphingans. However, there few reports on catalytic mechanism hydrolysate analysis SpnR. Here, SpnR from Sphingomonas sp. HT-1 was heterologously expressed in Escherichia coli. The optimal temperature pH purified were 35 °C 7.2, respectively. good alkali resistance relatively mild optimum temperature. Na+ Fe3+ increased activities, contrast Zn2+ EDTA. could degrade sphingans, most suitable substrate welan. Analysis cleavage pattern revealed that acts specifically β-1,4-glycosidic bond obtain minimum trisaccharide sphingan (SpnOS). gut microbiota vitro showed SpnOS caused substantial modification intestinal microbiota, characterized an augmented proliferation beneficial bacterial populations, notably Bacteroides Lactobacillus.

Language: Английский

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