Exploration of the Mechanism of Juglone's Anti-Colorectal Cancer Effects Based on Network Pharmacology and Experimental Validation DOI Creative Commons

Yunting DENG,

Yanan ZAHNG,

Jinhai Huo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Abstract Objectives This study aims to explore the mechanism of juglone's anticancer effects against colorectal cancer. The methods including network pharmacology prediction, molecular docking techniques, and cell experiments. Background Colorectal cancer originates from mucosal layer colon or rectum. It is third most common worldwide, accounting for approximately 10% all cases. also second leading cause cancerrelated deaths globally. Improving treatment outcomes survival rates urgently needed. Juglone exhibits antiinflammatory, antiviral, properties, has been used inhibit tumor growth with promising results. However, there limited experimental research investigating underlying mechanisms inhibition Methods Utilizing identify potential targets pathways juglone in combating Using techniques validate interaction between core Evaluating on proliferation/toxicity through experiments, employing Western blot assess expression levels target proteins, thereby elucidating action. Results We identified that anticolorectal involve 51 156 pathways. inhibits proliferation a dosedependent manner. results indicate suppresses development by modulating TP53 proteins. ConclusionsJuglone multipathway, multitarget growth, providing basis clinical

Language: Английский

Exploiting the Anticancer, Antimicrobial and Antiviral Potential of Naphthoquinone Derivatives: Recent Advances and Future Prospects DOI Creative Commons
Shouyan Xiang, Yubei Li, Shah Nawaz Khan

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 350 - 350

Published: Feb. 28, 2025

Cancer remains a primary cause of mortality, with over 18.1 million new cases and 9.6 deaths globally in 2018. Chemotherapy, which utilizes spectrum cytotoxic drugs targeting the rapidly dividing cancer cells, is predominant treatment modality. However, tendency chemotherapeutics to induce drug resistance exhibit non-specific cytotoxicity necessitates development anticancer agents heightened efficacy minimized toxicity. In recent years, discovery safe effective antibacterial/antiviral has also been hot spot medicinal chemistry. This paper comprehensively reviews synthesis, anticancer/antibacterial/antiviral activity, structure–activity relationships natural 1,4-naphthoquinones their derivatives. It highlights potential as efficient low-toxicity antitumor anti-infectious candidates.

Language: Английский

Citations

0
Discovery and biological evaluation of potent 2-trifluoromethyl acrylamide warhead-containing inhibitors of protein disulfide isomerase DOI

Jung-Chun Chu,

Keng‐Chang Tsai, Tingyu Wang

et al.

European Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 283, P. 117169 - 117169

Published: Dec. 16, 2024

Language: Английский

Citations

1
A comprehensive Study of Juglone's Effect on Polyphenol Oxidase in Cucumber: In Vitro Experiments and Computational Docking and Dynamics Insights DOI Creative Commons
İsmail Kocaçalışkan, Şenay Vural Korkut,

Emre Aktaş

et al.

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(4)

Published: July 1, 2024

Abstract This study explores the impact of juglone on cucumber ( Cucumis sativus cv . Beith Alpha), scrutinizing its effects seed germination, growth, and polyphenol oxidase (PPO) enzyme's activity gene expression. Employing concentrations ranging from 0.01 to 0.5 mM, we found juglone's be concentration‐dependent. At lower (0.01 0.1 mM), promoted root shoot growth along with whereas higher (0.25 mM) exerted inhibitory effects, delineating a threshold for allelopathic influence. Notably, PPO surged, especially at mM in roots, hinting oxidative stress involvement. Real‐time PCR unveiled that modulates expression cotyledons, peaking diminishing elevated levels. Correlation analyses elucidated positive link between juglone‐induced cotyledon but negative correlation heightened enzyme activity. Additionally, germination percentage inversely correlated activity, while activities positively associated dopa catechol substrates both roots cotyledons. Molecular docking studies revealed selective interactions PPO's B chain, suggesting regulatory impacts. Protein interaction assessments highlighted influence amino acid metabolism, molecular dynamics indicated stronger, more stable binding PPO, inferring potential alterations function stability. Conclusively, our findings elucidate dose‐dependent physiological biochemical shifts plants, offering insights into role plant response, metabolic modulation.

Language: Английский

Citations

0
Exploration of the Mechanism of Juglone's Anti-Colorectal Cancer Effects Based on Network Pharmacology and Experimental Validation DOI Creative Commons

Yunting DENG,

Yanan ZAHNG,

Jinhai Huo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Abstract Objectives This study aims to explore the mechanism of juglone's anticancer effects against colorectal cancer. The methods including network pharmacology prediction, molecular docking techniques, and cell experiments. Background Colorectal cancer originates from mucosal layer colon or rectum. It is third most common worldwide, accounting for approximately 10% all cases. also second leading cause cancerrelated deaths globally. Improving treatment outcomes survival rates urgently needed. Juglone exhibits antiinflammatory, antiviral, properties, has been used inhibit tumor growth with promising results. However, there limited experimental research investigating underlying mechanisms inhibition Methods Utilizing identify potential targets pathways juglone in combating Using techniques validate interaction between core Evaluating on proliferation/toxicity through experiments, employing Western blot assess expression levels target proteins, thereby elucidating action. Results We identified that anticolorectal involve 51 156 pathways. inhibits proliferation a dosedependent manner. results indicate suppresses development by modulating TP53 proteins. ConclusionsJuglone multipathway, multitarget growth, providing basis clinical

Language: Английский

Citations

0