Raman Spectroscopy in Analysis of Biomolecules
Encyclopedia of Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 38
Published: March 27, 2025
Abstract
Raman
spectroscopy
(
RS
)
is
a
vibrational
technique
based
on
the
phenomenon
of
inelastic
light
scattering
from
matter
(the
effect).
It
versatile
tool
for
probing
biological
events
at
molecular
level
and
identification
biomolecules.
The
characteristic
mark
modern
variety
techniques
used
to
explore
effect.
In
this
article,
advantages
limitations
different
methodologies
including
conventional
Raman,
resonance
RR
),
ultraviolet
UVRR
surface‐enhanced
SER
SERR
shell‐isolated
nanoparticle‐enhanced
(SHINER),
tip‐enhanced
(TER),
Fourier
transform
FT‐Raman
micro‐Raman,
time‐resolved
coherent
anti‐Stokes
CARS
spectroscopies
that
can
be
analysis
structure
function
biomolecules
are
discussed.
particularly
important
in
applications
since
chromophoric
groups
(active
centers
proteins,
aromatic
amino
acid
residues,
nucleotides),
which
many
cases
responsible
biomolecules,
probed
selectively
by
tuning
excitation
wavelength
electronic
absorption
transition
chromophore.
Surface‐enhanced
(SER
SERR)
most
sensitive,
allowing
single
interpretation
spectra
requires
spectra‐structure
correlations.
Numerous
marker
bands
conformation,
hydrogen
bonding,
protonation,
hydrophobic
interaction
main
constituents
biopolymers
provided
Language: Английский
Vibrational Self-Consistent Field (VSCF) and Post-VSCF Method Calculations Combined with the Reference Interaction Site Model Self-Consistent Field Method Coupled with the Constrained Spatial Electron Density Distribution: Applications to NaHCOO in Aqueous Phase
Journal of Chemical Theory and Computation,
Journal Year:
2024,
Volume and Issue:
20(11), P. 4885 - 4892
Published: May 30, 2024
Investigating
vibrational
behavior
in
solution
is
crucial
for
understanding
molecular
dynamics
within
a
solvent
environment.
Notably,
the
analysis
of
Raman
spectra
molecules
important
owing
to
its
ability
unveil
intricate
solute–solvent
interactions.
Previous
studies
have
effectively
employed
frequency
calculations
utilizing
reference
interaction
site
model
self-consistent
field
method
conjunction
with
constrained
spatial
electron
density
distribution
(RISM–SCF–cSED)
understand
vibrations
solution,
primarily
focusing
on
fundamental
modes.
However,
oversight
overtones
and
combination
tones
these
prompted
us
combine
(VSCF)
second-order
Mo̷ller–Plesset
perturbation
(VMP2)
methods
RISM–SCF–cSED
address
aspects
theoretically.
Illustrating
efficacy
this
integrated
approach,
we
computed
sodium
formate
(NaHCOO)
water,
revealing
necessity
accounting
anharmonicity
analysis.
Our
findings
underscore
potency
VSCF
VMP2
as
robust
theoretical
framework
such
calculations.
Language: Английский
Raman Analysis of Lipids in Cells: Current Applications and Future Prospects
Journal of Pharmaceutical Analysis,
Journal Year:
2024,
Volume and Issue:
15(4), P. 101136 - 101136
Published: Nov. 1, 2024
Lipids
play
an
important
role
in
the
regulation
of
cell
life
processes.
Although
there
are
various
lipid
detection
methods,
Raman
spectroscopy,
a
non-invasive
technique,
provides
detailed
chemical
composition
profiles
without
complex
sample
preparation
procedure
and
possesses
greater
potential
basic
biology,
clinical
diagnosis
disease
therapy.
In
this
review,
we
summarized
characteristics
advantages
Raman-based
techniques
their
primary
contribution
to
illustrating
cellular
metabolism.
Language: Английский