Identification of Phytosphingosine-Based 1-O-Acylceramide in Human Stratum Corneum and Investigation of Its Role in Skin Barrier
Cosmetics,
Journal Year:
2025,
Volume and Issue:
12(2), P. 47 - 47
Published: March 11, 2025
Phytosphingosine-based
1-O-acylceramide
(CerENP)
from
the
human
stratum
corneum
has
not
been
reported
on.
High-resolution
mass
spectrometry
was
used
to
identify
CerENP
skin
samples.
A
vehicle-controlled
study
performed
investigate
physiological
interaction
between
ceramide
NP
(CerNP)
and
with
respect
hydration,
cohesion,
TEWL,
all
of
which
were
measured.
Twenty
volunteers
treated
test
creams
containing
together
CerNP,
significantly
improved
barrier
parameters
after
four
weeks
application:
1.
Skin
hydration
increased
by
26%
compared
when
CerNP
alone,
moisture
retention
better
than
it
control.
2.
Stratum
(SC)
cohesion
strengthened
only
using
cream
formulated
CerENP.
The
results
suggested
boosting
effects
on
functions
exerted
since
a
small
amount
is
required,
as
low
one-tenth
CerNP.
This
first
report
identification
in
SC
its
activities
skin.
In
conclusion,
combinatorial
use
at
an
appropriate
relative
ratio
could
be
new
normal
developing
ideal
moisturizer
for
dry
atopic
Language: Английский
Biomimetic Multilayered Lipid Nanovesicles for Potent Protein Vaccination
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
13(18)
Published: June 8, 2024
Lipid
vesicles
are
widely
used
for
drug
and
gene
delivery,
but
their
structural
instability
reduces
in
vivo
efficacy
requires
specialized
handling.
To
address
these
limitations,
strategies
like
lipid
cross-linking
polymer-lipid
conjugation
suggested
to
enhance
stability
biological
efficacy.
However,
the
metabolism
of
altered
lipids
remains
unclear,
necessitating
further
studies.
A
new
stabilization
technique
without
chemical
modification
is
urgently
needed.
Here,
a
bio-mimetic
approach
fabricating
robust
multilamellar
delivery
protein
antigens
presented.
This
method
leverages
1-O-acylceramide,
natural
skin
lipid,
facilitate
self-assembly
nanovesicles.
Incorporating
anchoring
bilayers
akin
its
role
stratum
corneum,
provides
excellent
under
environmental
stresses,
including
freeze-thaw
cycles.
Encapsulating
ovalbumin
as
model
antigen
adjuvant
monophosphoryl
demonstrates
vesicle's
potential
nanovaccine
platform.
In
vitro
studies
show
enhanced
immune
responses
with
both
unilamellar
vesicles,
analyses
highlight
superior
efficiency
inducing
higher
antibody
cytokine
levels.
work
suggests
ceramide-induced
an
effective
platform
stability.
Language: Английский