In Vivo Ribosome-Amplified MetaBOlism, RAMBO, Effect Observed by Real Time Pulse Chase, RTPC, NMR Spectroscopy

Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 27, 2025

Quinary interactions between proteins and ribosomes play an important role in regulating biological activity through a phenomenon termed the Ribosome-Amplified MetaBOlism, RAMBO, effect. This effect has been documented vitro but not vivo. Real time pulse chase, RTPC, NMR spectroscopy, coupled with isotopic flux analysis Escherichia coli was used to validate RAMBO The ribosomal-targeting antibiotic chloramphenicol employed disrupt quinary structure of pyruvate kinase, final enzyme glycolysis. Kinetic profiling demonstrated that deactivation by also observed vivo, thereby confirming potential noninvasive modular design RTPC-NMR platform allows for high-resolution metabolic monitoring across different cell types, providing broad applicability studying real-time responses external stimuli living cells.

Language: Английский

Electrical Stimulation Therapy – Dedicated to the Perfect Plastic Repair

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Tissue repair and reconstruction are a clinical difficulty. Bioelectricity has been identified as critical factor in supporting tissue cell viability during the process, presenting substantial potential for application. This review delves into various sources of electrical stimulation identifies appropriate electrode materials use. It also highlights biological mechanisms at both subcellular cellular levels, elucidating how these interactions facilitate regeneration processes across different organs. Moreover, specific outlined, detailing their impact on activity. The future development trends projected from two perspectives: optimization equipment performance fulfillment demands, focusing feasibility, safety, cost-effectiveness technologies.

Language: Английский