The response to desiccation in Acinetobacter baumannii DOI Creative Commons
Massimiliano Lucidi, Giulia Capecchi,

Cinzia Spagnoli

et al.

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 12, 2025

The long-term resistance to desiccation on abiotic surfaces is a key determinant of the adaptive success Acinetobacter baumannii as healthcare-associated bacterial pathogen. Here, cellular and molecular mechanisms enabling A. resist persist were investigated. Experiments set up mimic response air-drying that would occur when cells contaminate fomites in hospitals. Resistance transition "viable but nonculturable" (VBNC) state determined laboratory-adapted strain ATCC 19606T epidemic ACICU. Culturability, membrane integrity, metabolic activity, virulence, gene expression profile compared between two strains at different stages desiccation. Upon desiccation, ACICU lose culturability lower their metabolism, enter VBNC state. However, desiccated fully recover virulence an insect infection model following rehydration physiological buffers or human biological fluids. Transcriptome chemical analyses during unveiled production protective metabolites (L-cysteine L-glutamate) decreased energetic metabolism consequent activation glyoxylate shunt (GS) pathway, confirmed by reduced resuscitation efficiency aceA mutants, lacking enzyme GS pathway. cell formation extensive reprogramming provide basis for with implications environmental control measures aimed preventing transmission

Language: Английский

The response to desiccation in Acinetobacter baumannii DOI Creative Commons
Massimiliano Lucidi, Giulia Capecchi,

Cinzia Spagnoli

et al.

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 12, 2025

The long-term resistance to desiccation on abiotic surfaces is a key determinant of the adaptive success Acinetobacter baumannii as healthcare-associated bacterial pathogen. Here, cellular and molecular mechanisms enabling A. resist persist were investigated. Experiments set up mimic response air-drying that would occur when cells contaminate fomites in hospitals. Resistance transition "viable but nonculturable" (VBNC) state determined laboratory-adapted strain ATCC 19606T epidemic ACICU. Culturability, membrane integrity, metabolic activity, virulence, gene expression profile compared between two strains at different stages desiccation. Upon desiccation, ACICU lose culturability lower their metabolism, enter VBNC state. However, desiccated fully recover virulence an insect infection model following rehydration physiological buffers or human biological fluids. Transcriptome chemical analyses during unveiled production protective metabolites (L-cysteine L-glutamate) decreased energetic metabolism consequent activation glyoxylate shunt (GS) pathway, confirmed by reduced resuscitation efficiency aceA mutants, lacking enzyme GS pathway. cell formation extensive reprogramming provide basis for with implications environmental control measures aimed preventing transmission

Language: Английский

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