Optimization of Capillary Vibrating Sharp-Edge Spray Ionization for Native Mass Spectrometry of Triplex DNA DOI Creative Commons

Sultan Mahmud,

Vikum K. Dewasurendra, Chandrima Banerjee

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Capillary vibrating sharp-edge spray ionization (cVSSI) has been used to study the effects of applied voltage and mass spectrometer heated inlet transfer tube temperature on DNA triplex ion production for native spectrometry (MS) samples. Overall, medium (-900 -1000 V) results in better desired ions (Tri) (i.e., those without cation adducts such as NH4 +, Na+, K+); spectral peak intensities [Tri]8-, [Tri]9-, [Tri]10- increase by ∼70, ∼260, ∼125 fold, respectively, compared higher voltages (-1100 -1500 V). The latter result increased adduct (Tri + ad) formation; 8-, 9-, 10- charge states; ratios Tri Tri+ad abundances ∼6 fold lower voltage. By capillary temperatures 300 400 °C, reach maximum values 6.1 × 105 ([Tri]8-), 2.9 106 ([Tri]9-), 6.4 ([Tri]10-). Ion respective species decrease ∼4, ∼14, ∼190 at a 450 °C. generally follow similar trend function with exception that are obtained 250 fragment (Tri-fr) °C resulting from excessive, in-source activation. From these studies, optimal MS large oligonucleotides cVSSI is 350 should be maintained ∼ -900 V. These studies lay foundation oligonucleotide negative-ion mode exploiting sensitivity enhancements cVSSI.

Language: Английский

Optimization of Capillary Vibrating Sharp-Edge Spray Ionization for Native Mass Spectrometry of Triplex DNA DOI Creative Commons

Sultan Mahmud,

Vikum K. Dewasurendra, Chandrima Banerjee

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Capillary vibrating sharp-edge spray ionization (cVSSI) has been used to study the effects of applied voltage and mass spectrometer heated inlet transfer tube temperature on DNA triplex ion production for native spectrometry (MS) samples. Overall, medium (-900 -1000 V) results in better desired ions (Tri) (i.e., those without cation adducts such as NH4 +, Na+, K+); spectral peak intensities [Tri]8-, [Tri]9-, [Tri]10- increase by ∼70, ∼260, ∼125 fold, respectively, compared higher voltages (-1100 -1500 V). The latter result increased adduct (Tri + ad) formation; 8-, 9-, 10- charge states; ratios Tri Tri+ad abundances ∼6 fold lower voltage. By capillary temperatures 300 400 °C, reach maximum values 6.1 × 105 ([Tri]8-), 2.9 106 ([Tri]9-), 6.4 ([Tri]10-). Ion respective species decrease ∼4, ∼14, ∼190 at a 450 °C. generally follow similar trend function with exception that are obtained 250 fragment (Tri-fr) °C resulting from excessive, in-source activation. From these studies, optimal MS large oligonucleotides cVSSI is 350 should be maintained ∼ -900 V. These studies lay foundation oligonucleotide negative-ion mode exploiting sensitivity enhancements cVSSI.

Language: Английский

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