Interactions of Cubosomes with Cell Membrane Lipids: Revealed through Cubic Membrane Property Analysis DOI

Ward Wakileh,

Nozomi Morishita Watanabe,

Zachary Nicolella

et al.

Langmuir, Journal Year: 2025, Volume and Issue: 41(17), P. 10808 - 10817

Published: April 28, 2025

Leveraged for their large drug payloads and fusogenic properties, cubosomes are promising platforms therapeutic applications. However, research regarding the properties of cubic membranes interactions with cell is still lacking. The study these an important factor to consider in design process due influence on cubosome structure capability release. To address this gap, we demonstrate effectiveness utilization membrane-bound fluorescence probes examine interaction behavior. First, demonstrated cubosomal membrane changes increasing concentration DOPC using steady-state time-resolved Laurdan DPH probes. generalized polarization (GP340) anisotropy (r) showed that well-hydrated fluidic transitioned into less hydrated states, transition lamellar phase. Finally, verified results first part by examining MO liposomes small-angle X-ray scattering (TR-SAXS) GP340 values probed over time. cubic-to-lamellar phase was confirmed from TR-SAXS spectra. By MO/DOPC compositions as a calibration curve, kinetics apparent lipid exchange (kapp) were obtained, amount course could be estimated. approach developed work showcases applications such estimation other types carriers. In addition, obtained provide deeper understanding relation between carrier model membranes. Such insights may prove valuable

Language: Английский

Interactions of Cubosomes with Cell Membrane Lipids: Revealed through Cubic Membrane Property Analysis DOI

Ward Wakileh,

Nozomi Morishita Watanabe,

Zachary Nicolella

et al.

Langmuir, Journal Year: 2025, Volume and Issue: 41(17), P. 10808 - 10817

Published: April 28, 2025

Leveraged for their large drug payloads and fusogenic properties, cubosomes are promising platforms therapeutic applications. However, research regarding the properties of cubic membranes interactions with cell is still lacking. The study these an important factor to consider in design process due influence on cubosome structure capability release. To address this gap, we demonstrate effectiveness utilization membrane-bound fluorescence probes examine interaction behavior. First, demonstrated cubosomal membrane changes increasing concentration DOPC using steady-state time-resolved Laurdan DPH probes. generalized polarization (GP340) anisotropy (r) showed that well-hydrated fluidic transitioned into less hydrated states, transition lamellar phase. Finally, verified results first part by examining MO liposomes small-angle X-ray scattering (TR-SAXS) GP340 values probed over time. cubic-to-lamellar phase was confirmed from TR-SAXS spectra. By MO/DOPC compositions as a calibration curve, kinetics apparent lipid exchange (kapp) were obtained, amount course could be estimated. approach developed work showcases applications such estimation other types carriers. In addition, obtained provide deeper understanding relation between carrier model membranes. Such insights may prove valuable

Language: Английский

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