The role of polyethylenimine-functionalized gold nanoclusters carrying plasmid CMTM5 in impeding the malignant progression of prostate cancer cells by promoting EGFR endocytosis DOI Open Access

Linjin Li,

Chengpeng Li,

Feilong Miao

et al.

Neoplasma, Journal Year: 2024, Volume and Issue: 71(01), P. 48 - 59

Published: Jan. 1, 2024

In this research, polyethylenimine-functionalized gold nanoclusters (PEI-AuNCs) were synthesized for the delivery of plasmid CMTM5 (pCMTM5) to prostate cancer (PCa) cells, with objective elucidating mechanism underlying its anticancer efficacy.The PEI-AuNCs loaded pCMTM5 (PEI-AuNCs@pCMTM5) tumor-targeting drug system was established.Subsequently, both obtained and PEI-AuNCs@pCMTM5 underwent characterization through a transmission electron microscope (TEM) dynamic light scattering (DLS).Employing RT-qPCR, western blot, flow cytometry, immunofluorescence, co-immunoprecipitation (Co-IP) assays, consequences overexpression on expression EGFR investigated.Moreover, influence PC-3 cells assessed CCK-8, wound healing assay, Transwell experiments.As result, presented as uniformly dispersed spherical stable particle sizes positive charges, showcasing favorable dispersion within solution.In comparison Lip2000, demonstrated superior transfection efficiency lower cellular toxicity.Following CMTM5, proliferative capacity markedly suppressed, while migratory invasive abilities exhibited noteworthy reduction, efficacy consistently outperforming that free pCMTM5.Subsequent mechanistic investigations unveiled does not directly inhibit synthesis or facilitate degradation, but r ather influences endocytic process EGFR.In conclusion, nano-delivery exhibits good biocompatibility efficaciously conveys PCa cells.Crucially, interact EGFR, governs ma lignant progression PC3 by promoting endocytosis.Key words:

Language: Английский

A comprehensive review of atomically precise metal nanoclusters with emergent photophysical properties towards diverse applications DOI
Subarna Maity, Sarita Kolay, Sikta Chakraborty

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Ligand-protected metal nanoclusters are an emerging class of nanomaterials at the interface atomic and plasmonic regimes with unique photophysical electrochemical properties, leading to diverse applications.

Language: Английский

Citations

5
Recent Advances in Long-Lived Emission in Coinage Metal Nanoclusters: Implications for Optoelectronic Applications DOI
Shagun Sharma, Kush Kaushik, Abdul Salam

et al.

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 7(1), P. 32 - 60

Published: Dec. 29, 2023

The emerging research area of ultrasmall, luminescent coinage metal nanoclusters exhibiting delayed emission through thermally-activated fluorescence (TADF) and room temperature phosphorescence (RTP) has garnered considerable interest in diverse applications such as imaging, therapy, optoelectronics. To utilize the for advanced applications, it is crucial to understand excited state dynamics governing emission. Here, we comprehensively review recent advancement design strategies aimed at achieving control over their long-lived luminescence. include solvent-induced aggregation, crystallization-induced self-assembly, complexation methods, ligand engineering, core doping, composition regulation. Through these strategies, can be achieved which provide TADF RTP luminescence mechanism. Our primary goal harness versatile capabilities advance optoelectronic properties broaden range practical applications.

Language: Английский

Citations

10
Revisiting protein protected gold and silver nanoclusters: Excited state dynamics and long-lived emission DOI Creative Commons
Shagun Sharma, Subhadeep Das, Kush Kaushik

et al.

Nanoscale, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Time-resolved luminescence spectroscopy unveiled the distinct excited state dynamics of protein-conjugated gold and silver nanoclusters.

Language: Английский

Citations

0
Dual emissive optically active gold nanoclusters endowed with circularly polarized phosphorescence DOI Creative Commons
Camelia Dutta, Sonia Maniappan, Jatish Kumar

et al.

Chemical Communications, Journal Year: 2023, Volume and Issue: 59(92), P. 13735 - 13738

Published: Jan. 1, 2023

Enantiomerically pure Au nanoclusters exhibiting dual-emission corresponding to fluorescence and phosphorescence were synthesized by adopting a facile approach. Chiral luminescence was observed for the triplet emission leading circularly polarized phosphorescence, both in solution solid states. The exhibited aggregation induced emission, aggregated clusters chiral phosphorescence.

Language: Английский

Citations

5
Room-Temperature Persistent Phosphorescence of Aggregated Gold Nanoclusters under Molecular Crystal Confinements DOI
Arin Bhakat, Ujjala Dey, Arun Chattopadhyay

et al.

The Journal of Physical Chemistry Letters, Journal Year: 2024, Volume and Issue: 15(32), P. 8151 - 8160

Published: Aug. 2, 2024

We report color-tunable and solvent-processable persistent fluorescence to phosphorescence switching at room temperature by doping gold nanoclusters (AuNCs) inside molecular crystals. This provides a significant insight into the tunability of photoluminescence property dopant depending on crystal environment compactness confinement, with possibility energy transfer from aggregated AuNCs. For test cases, we have doped histidine-stabilized AuNCs (HIS-AuNCs) histidine (HIS-AuNCs-HIS) isophthalic acid (HIS-AuNCs-IPA) crystals, respectively, glutathione-stabilized (GSH-AuNCs) crystals (GSH-AuNCs-HIS). The maximal decay time recorded for was 9.38 ms, quantum yield value measured as 25%. possible states potential interactions between NCs host were accounted through density functional theory calculations docking techniques, respectively. finding opens new possibilities designing producing AuNC-based luminous multilayer information encryption, display, biological applications.

Language: Английский

Citations

1
Photochemical Eco-Friendly Synthesis of Photothermal and Emissive Copper Nanoclusters in Water: Towards Sustainable Nanomaterials DOI Creative Commons
Angelo Ferlazzo,

Stefano Bonforte,

Federica Florio

et al.

Materials Advances, Journal Year: 2024, Volume and Issue: 5(20), P. 8034 - 8041

Published: Jan. 1, 2024

We performed a sustainable and environmentally benign synthesis of stable Cu nanoclusters in water by UV irradiation II complex.

Language: Английский

Citations

0
Assessing the overflowing pile-up effect on the photoluminescence lifetime of nanomaterials DOI
Shagun Sharma, Aditya Yadav, Kush Kaushik

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The tunable complex emissive states with nanosecond to microsecond lifetimes in nanomaterials, arise due their structural heterogeneity, enabling them a wide range of advanced optoelectronic applications. However, understanding the photoluminescence lifetime these nanomaterials is critically challenged by overflowing pile-up effect, which occurs high repetition rate light source time-correlated single photon counting (TCSPC) technique. Here, we provide quantitative analysis, especially metal nanoclusters, complexes, and semiconductor quantum dots, suggests that same experimental parameters can mislead data interpretation for long-ranged luminescent nanomaterials. We demonstrate effect could be fatal while analyzing excited state lifetime. Furthermore, optimized used get actual samples. hope our findings will crucial obtaining error-free accurate dynamics long-range

Language: Английский

Citations

0
A smartphone-assisted ratiometric fluorescence sensor based on dual-emission copper nanoclusters for visual detection lead ion DOI
Bo Peng, Mingyu Li,

Huijing Chen

et al.

Dyes and Pigments, Journal Year: 2024, Volume and Issue: unknown, P. 112581 - 112581

Published: Dec. 1, 2024

Language: Английский

Citations

0
The role of polyethylenimine-functionalized gold nanoclusters carrying plasmid CMTM5 in impeding the malignant progression of prostate cancer cells by promoting EGFR endocytosis DOI Open Access

Linjin Li,

Chengpeng Li,

Feilong Miao

et al.

Neoplasma, Journal Year: 2024, Volume and Issue: 71(01), P. 48 - 59

Published: Jan. 1, 2024

In this research, polyethylenimine-functionalized gold nanoclusters (PEI-AuNCs) were synthesized for the delivery of plasmid CMTM5 (pCMTM5) to prostate cancer (PCa) cells, with objective elucidating mechanism underlying its anticancer efficacy.The PEI-AuNCs loaded pCMTM5 (PEI-AuNCs@pCMTM5) tumor-targeting drug system was established.Subsequently, both obtained and PEI-AuNCs@pCMTM5 underwent characterization through a transmission electron microscope (TEM) dynamic light scattering (DLS).Employing RT-qPCR, western blot, flow cytometry, immunofluorescence, co-immunoprecipitation (Co-IP) assays, consequences overexpression on expression EGFR investigated.Moreover, influence PC-3 cells assessed CCK-8, wound healing assay, Transwell experiments.As result, presented as uniformly dispersed spherical stable particle sizes positive charges, showcasing favorable dispersion within solution.In comparison Lip2000, demonstrated superior transfection efficiency lower cellular toxicity.Following CMTM5, proliferative capacity markedly suppressed, while migratory invasive abilities exhibited noteworthy reduction, efficacy consistently outperforming that free pCMTM5.Subsequent mechanistic investigations unveiled does not directly inhibit synthesis or facilitate degradation, but r ather influences endocytic process EGFR.In conclusion, nano-delivery exhibits good biocompatibility efficaciously conveys PCa cells.Crucially, interact EGFR, governs ma lignant progression PC3 by promoting endocytosis.Key words:

Language: Английский

Citations

0