ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Excess accumulation of misfolded and mutated human lysozyme (HuL) is the pathological hallmark non-neuropathic systemic amyloidosis. These deposits are rich in cross β-sheet conformers often exist as polymorphic fibrillar structures, which makes it a tricky challenging task to design therapeutic interventions toward HuL-linked amyloidopathy. Here we aimed an effective antiamyloid metal nanoparticle formulation target exposed hydrophobic aggregation-prone stretches HuL. Initially, synthesized characterized piperine-coated gold nanoparticles (AuNPsPip). ThT-probed aggregation studies HuL presence absence AuNPsPip revealed inhibition aggregation. This effect was confirmed through dynamic light scattering (DLS) fluorescence microscopy analyses. We further investigated whether could bind preformed fibrils prevent secondary nucleation process, crucial step amyloidogenesis. Our results showed that not only prevented seed-induced but also disassembled amyloid aggregates, observed with AuNPs or piperine. Experimental computational suggest retention native structure ability interact residues key factors mechanism. The findings this work may aid developing nanoparticle-based formulations pathologies linked

Language: Английский