Electrostatics of Salt-Dependent Reentrant Phase Behaviors Highlights Diverse Roles of ATP in Biomolecular Condensates DOI Open Access
Yi‐Hsuan Lin, Tae Hun Kim, Suman Das

et al.

Published: Dec. 30, 2024

Liquid-liquid phase separation (LLPS) involving intrinsically disordered protein regions (IDRs) is a major physical mechanism for biological membraneless compartmentalization. The multifaceted electrostatic effects in these biomolecular condensates are exemplified here by experimental and theoretical investigations of the different salt- ATP-dependent LLPSs an IDR messenger RNA-regulating Caprinl its phosphorylated variant pY-Caprinl, exhibiting, e.g., reentrant behaviors some instances but not others. Experimental data rationalized modeling using analytical theory, molecular dynamics, polymer field-theoretic simulations, indicating that interchain ion bridges enhance LLPS polyelectrolytes such as high valency ATP-magnesium significant factor colocalization with condensed phases, similar trends observed other IDRs. nature features complements ATP’s involvement π-related interactions amphiphilic hydrotrope, underscoring general role modulating concentrations functional ramifications.

Language: Английский

Electrostatics of salt-dependent reentrant phase behaviors highlights diverse roles of ATP in biomolecular condensates DOI Creative Commons
Yi‐Hsuan Lin, Tae Hun Kim, Suman Das

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: March 3, 2025

Liquid-liquid phase separation (LLPS) involving intrinsically disordered protein regions (IDRs) is a major physical mechanism for biological membraneless compartmentalization. The multifaceted electrostatic effects in these biomolecular condensates are exemplified here by experimental and theoretical investigations of the different salt- ATP-dependent LLPSs an IDR messenger RNA-regulating Caprin1 its phosphorylated variant pY-Caprin1, exhibiting, example, reentrant behaviors some instances but not others. Experimental data rationalized modeling using analytical theory, molecular dynamics, polymer field-theoretic simulations, indicating that interchain ion bridges enhance LLPS polyelectrolytes such as high valency ATP-magnesium significant factor colocalization with condensed phases, similar trends observed other IDRs. nature features complements ATP’s involvement π-related interactions amphiphilic hydrotrope, underscoring general role modulating concentrations functional ramifications.

Language: Английский

Citations

2
Electrostatics of Salt-Dependent Reentrant Phase Behaviors Highlights Diverse Roles of ATP in Biomolecular Condensates DOI Creative Commons
Yi‐Hsuan Lin, Tae Hun Kim, Suman Das

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Sept. 5, 2024

Liquid-liquid phase separation (LLPS) involving intrinsically disordered protein regions (IDRs) is a major physical mechanism for biological membraneless compartmentalization. The multifaceted electrostatic effects in these biomolecular condensates are exemplified here by experimental and theoretical investigations of the different salt- ATP-dependent LLPSs an IDR messenger RNA-regulating Caprin1 its phosphorylated variant pY-Caprin1, exhibiting, example, reentrant behaviors some instances but not others. Experimental data rationalized modeling using analytical theory, molecular dynamics, polymer field-theoretic simulations, indicating that interchain ion bridges enhance LLPS polyelectrolytes such as high valency ATP-magnesium significant factor colocalization with condensed phases, similar trends observed other IDRs. nature features complements ATP’s involvement π-related interactions amphiphilic hydrotrope, underscoring general role modulating concentrations functional ramifications.

Language: Английский

Citations

2
Electrostatics of Salt-Dependent Reentrant Phase Behaviors Highlights Diverse Roles of ATP in Biomolecular Condensates DOI Open Access
Yi‐Hsuan Lin, Tae Hun Kim, Suman Das

et al.

Published: Dec. 30, 2024

Liquid-liquid phase separation (LLPS) involving intrinsically disordered protein regions (IDRs) is a major physical mechanism for biological membraneless compartmentalization. The multifaceted electrostatic effects in these biomolecular condensates are exemplified here by experimental and theoretical investigations of the different salt- ATP-dependent LLPSs an IDR messenger RNA-regulating Caprinl its phosphorylated variant pY-Caprinl, exhibiting, e.g., reentrant behaviors some instances but not others. Experimental data rationalized modeling using analytical theory, molecular dynamics, polymer field-theoretic simulations, indicating that interchain ion bridges enhance LLPS polyelectrolytes such as high valency ATP-magnesium significant factor colocalization with condensed phases, similar trends observed other IDRs. nature features complements ATP’s involvement π-related interactions amphiphilic hydrotrope, underscoring general role modulating concentrations functional ramifications.

Language: Английский

Citations

0