Thioflavin T Lasing Probe for Mucin Detection in Simulated Tears as a Targeting Strategy for Brain Tumors DOI Creative Commons

Ewelina Jalonicka,

Konstantin Rusakov, Grzegorz Szwachta

et al.

ACS Chemical Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

A novel optical approach for a noninvasive detection of mucins in tear fluid is proposed, aiming at the early diagnosis brain tumors such as glioblastoma. Utilizing Thioflavin T (ThT) fluorescent probe, our study demonstrates that ThT selectively binds to (modeled by MUC3) DEMI water, artificial tears, and simulated tears. Steady-state time-resolved fluorescence spectroscopy reveal mucin binding induces significant enhancement prolonged emission lifetime, indicative restricted intramolecular rotation. Importantly, application Fabry-Pérot cavity lasing enabled resolution distinct spectral signatures ThT-mucin complex, including emergence dual peaks an increased threshold mucin-rich samples compared controls. These fingerprints provide compelling evidence specific interactions are not discernible with conventional techniques. Our findings highlight potential probe method sensitive, platform detecting offering promising strategy

Language: Английский

Transient Absorption Microscopy for Early-Stage Imaging of Protein Aggregates Using Thioflavin T DOI Creative Commons
Przemysław Słota, Piotr Fita

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

We present the design and application of a transient absorption microscope for imaging protein aggregates at early stages growth, utilizing widely used amyloid marker dye Thioflavin T (ThT). By employing femtosecond laser pulses excitation probing, distinguishes signals from free ThT molecules bound to aggregates, enabling different development. Spatially resolved measurements revealed two distinct excited-state lifetimes in presence insulin aggregates: short lifetime (approximately 10 ps) longer (in range 100–200 ps). The dominates aggregation, reflecting binding small, disordered or amorphous precursors. As aggregation progresses, contribution increases, corresponding formation larger, more ordered structures. This observation agrees with theoretical models formation, where serve as nucleation sites growth mature fibrils. results demonstrate capability microscopy resolve spatial temporal dynamics aggregation. approach offers potential applications study amyloid-related diseases, including characterization inhibitors pathological deposits tissue samples.

Language: Английский

Citations

1
Thioflavin T Lasing Probe for Mucin Detection in Simulated Tears as a Targeting Strategy for Brain Tumors DOI Creative Commons

Ewelina Jalonicka,

Konstantin Rusakov, Grzegorz Szwachta

et al.

ACS Chemical Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

A novel optical approach for a noninvasive detection of mucins in tear fluid is proposed, aiming at the early diagnosis brain tumors such as glioblastoma. Utilizing Thioflavin T (ThT) fluorescent probe, our study demonstrates that ThT selectively binds to (modeled by MUC3) DEMI water, artificial tears, and simulated tears. Steady-state time-resolved fluorescence spectroscopy reveal mucin binding induces significant enhancement prolonged emission lifetime, indicative restricted intramolecular rotation. Importantly, application Fabry-Pérot cavity lasing enabled resolution distinct spectral signatures ThT-mucin complex, including emergence dual peaks an increased threshold mucin-rich samples compared controls. These fingerprints provide compelling evidence specific interactions are not discernible with conventional techniques. Our findings highlight potential probe method sensitive, platform detecting offering promising strategy

Language: Английский

Citations

0