How Transcriptional Bursting and mRNA Production Affect Precise Timing of Cell Lysis Phenomena DOI
Zhiyuan Lyu, Anupam Mondal, Anatoly B. Kolomeisky

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Bacterial viruses infect bacterial cells and stimulate the production of holin proteins that accumulate in cellular membranes. When number such reaches a threshold, membrane permeabilizes cell is destroyed process known as lysis. Experimental studies indicate lysis occurs at specific times, although underlying molecular mechanisms precise timing remain not well understood. Recently, theoretical framework has been introduced to explain these phenomena coupling between stochastic processes holins accumulation breaking leads threshold behavior. However, this approach does account for many biologically important In work, we investigated role transcriptional bursting mRNA on dynamics The original extended, allowing us evaluate under more realistic biological conditions using analytical calculations Monte Carlo computer simulations. It shown explicitly random transcription do affect threshold-like lysis, they influence absolute values maximal thresholds their distributions. also found effect generally stronger than due bursting. Physical-chemical arguments observations are presented. Thus, our analysis suggests robust phenomenon despite involving multiple biochemical processes. Our clarifies some mechanistic aspects complex

Language: Английский

Dynamic interactions of retroviral Gag condensates with nascent viral RNA at transcriptional burst sites: implications for genomic RNA packaging DOI Creative Commons
Rebecca J. Kaddis Maldonado, Leslie J. Parent

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

Abstract Retroviruses are responsible for significant pathology in humans and animals, including the acquired immunodeficiency syndrome a wide range of malignancies. A crucial yet poorly understood step replication cycle is recognition selection unspliced viral RNA (USvRNA) by retroviral Gag protein, which binds to psi (Ψ) packaging sequence 5’ leader, package it as genomic (gRNA) into nascent virions. It was previously thought that initially bound gRNA cytoplasm. However, previous studies demonstrated Rous sarcoma virus (RSV) protein traffics transiently through nucleus, necessary efficient packaging. These data formed strong premise hypothesis selects at transcription sites location highest concentration USvRNA molecules cell. In support this model, using fixed cells infected with RSV revealed co-localizes large nuclear foci representing transcriptional burst sites. To test idea, we used single molecule labeling imaging techniques visualize fluorescently-tagged, actively transcribing genomes, proteins living cells. condensates were observed co-localized sites, forming co- localized ribonucleoprotein complexes (vRNPs). results novel paradigm assembly interacts dynamic kissing interaction capture incorporation

Language: Английский

Citations

0
How Transcriptional Bursting and mRNA Production Affect Precise Timing of Cell Lysis Phenomena DOI
Zhiyuan Lyu, Anupam Mondal, Anatoly B. Kolomeisky

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Bacterial viruses infect bacterial cells and stimulate the production of holin proteins that accumulate in cellular membranes. When number such reaches a threshold, membrane permeabilizes cell is destroyed process known as lysis. Experimental studies indicate lysis occurs at specific times, although underlying molecular mechanisms precise timing remain not well understood. Recently, theoretical framework has been introduced to explain these phenomena coupling between stochastic processes holins accumulation breaking leads threshold behavior. However, this approach does account for many biologically important In work, we investigated role transcriptional bursting mRNA on dynamics The original extended, allowing us evaluate under more realistic biological conditions using analytical calculations Monte Carlo computer simulations. It shown explicitly random transcription do affect threshold-like lysis, they influence absolute values maximal thresholds their distributions. also found effect generally stronger than due bursting. Physical-chemical arguments observations are presented. Thus, our analysis suggests robust phenomenon despite involving multiple biochemical processes. Our clarifies some mechanistic aspects complex

Language: Английский

Citations

0