Kinetics of hydrogen migration and string of conformation transformation for pyruvic acid under bioelectric stimulation
Abstract
Context
The
competition
between
the
proton
migration
and
conformational
transformation
of
biomolecules
stimulated
by
bioelectric
currents
is
importance
for
maintaining
normal
biochemical
reaction
process
organisms.
To
reveal
mechanism
pyruvic
acid
under
currents,
effect
external
electric
field
on
bi-proton
cooperativity
dimer
was
investigated,
accompanied
a
comparison
with
finite
temperature
string
(FTS)
enhanced
sampling
molecular
transformation.
results
indicate
that
influences
fields
along
x -
y -axis
directions
transition
state
are
more
significant
than
those
z -axis
direction,
x -axis
direction
most
significant.
induced
+
x-
or
y -
tends
to
form
products
from
in
which
hydroxyl
group
same
side
as
‒CH3
‒CF3
group.
There
some
good
linear
relationships
bond
length
changes,
atoms
molecules
(AIM)
surface
electrostatic
potential
statistical
changes
state,
barrier
free
energy
strengths.
spatial
order
parameters
(φ ,
ψ )
can
be
quickly
converged
through
umbrella
parameter
averaging,
thereby
obtaining
mean
force
(PMF)
Under
bioelectricity,
activation
energies
double
much
greater
PMF
barriers
its
transformation,
indicating
stimulation
majority
undergo
rather
migration.
bioelectricity
greatly
affects
migration,
while
it
has
little
molecules.
This
study
important
theoretical
significance
scientific
value
further
investigations
processes
sports
human
science.
Methods
effects
pathway,
barrier,
rate
constant
were
studied
using
M06-2X/6-311
G**,
M06-2X/aug-cc-pVTZ
CCSD(T)/6-311
G(2d,p)
methods
Gaussian
09
packages.
A
chaotic
FTS
model
constructed,
pyruvate
CHARMM22
NAMD
software
packages,
PLUMED
program.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 3, 2025
Language: Английский