A Hollow Amorphous Bimetal Organic Framework for Synergistic Cuproptosis/Ferroptosis/Apoptosis Anticancer Therapy via Disrupting Intracellular Redox Homeostasis and Copper/Iron Metabolisms

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(40)

Published: July 30, 2022

Abstract Cuproptosis is a very newly recognized regulated cell death modality that distinct from known mechanisms and shows enormous prospect in cancer treatment. However, its efficacy copper‐dependent restricted by strictly copper metabolism. Herein, novel copper/iron hybrid hollow amorphous metal organic framework (HaMOF) developed as an oxidative stress amplifier metabolic disrupter for synergistic cuproptosis/ferroptosis/apoptosis anticancer therapy. The HaMOF fabricated Cu 2+ , 3,3′‐dithiobis(propionohydrazide) Fe 3+ via unsaturated coordination‐etching integration strategy, then doxorubicin loaded followed surface decoration with hyaluronan. obtained DOX@Fe/CuTH exhibits tumor microenvironment‐triggered catalytic therapeutic property, wherein it can amplify cellular simultaneously boosting H 2 O production depleting glutathione. Moreover, cause mitochondrial dysfunction downregulate the expressions of transporter ATP7A iron FPN 1, thereby leading to disorders high retentions cytoplasm •OH generation. overloaded lipoylated protein dihydrolipoamide S‐acetyltransferase aggregation lead cuproptosis. Collectively, both augmented induce potent ferroptosis, which synergizes cuproptosis DOX‐mediated apoptosis efficiently suppress growth. This bimetallic nanoplatform provides new paradigm boost cuproptosis‐related therapies.

Language: Английский

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Amorphous metal–organic frameworks: Pioneering frontiers in cancer diagnostics and therapeutics

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 492, P. 152295 - 152295

Published: May 16, 2024

The relentless advancement of cancer, a leading cause mortality worldwide, underscores the need for innovative therapeutic strategies. Amorphous metal–organic frameworks (aMOFs) have emerged as paradigm-shifting solution in field cancer diagnostics and therapeutics, distinguishing themselves from their crystalline counterparts through superior properties functionalities. This comprehensive review explores synthesis, unique properties, multifaceted applications aMOFs diagnosis treatment. Compared with counterparts, display including enhanced catalytic performance, exceptional porosity, elevated thermal conductivity. These underpin extensive use sensitive biochemical detection precision drug delivery, tumor ablation, other applications. Their inherent chemical tunability further enables integration multifunctional anticancer strategies, ranging multiple biomedical imaging to phototherapy metal ion-mediated apoptosis, positioning versatile theranostic nanoplatforms. article delves into current oncological challenges prospects aMOFs, underscoring transformative potential advanced diagnostics, targeted therapy, development next-generation convergence computational simulations interdisciplinary research is anticipated accelerate clinical translation heralding new era personalized medicine.

Language: Английский

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Hollow Hierarchical Porous and Antihydrolytic Spherical Zeolitic Imidazolate Frameworks for Enzyme Encapsulation and Biocatalysis

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(7), P. 9466 - 9482

Published: Feb. 7, 2024

The creation of a new metal-organic framework (MOF) with hollow hierarchical porous structure has gained significant attention in the realm enzyme immobilization. present work employed novel, facile, and effective combinatorial technique to synthesize modified MOF (N-PVP/HZIF-8) hierarchically core-shell structure, allowing for preservation structural integrity encapsulated molecules. Scanning electron microscopy, X-ray diffraction, photoelectron spectroscopy, confocal laser scanning other characterization tools were used fully explore changes morphological surface properties different stages preparation immobilization CRL-N-PVP/HZIF-8, thus showing superiority N-PVP/HZIF-8 as an platform logic process on carrier. Additionally, maximum loading was 216.3 mg mL

Language: Английский

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Metal-organic frameworks: synthesis, properties, wound dressing, challenges and scopes in advanced wound dressing

Biomedical Materials, Journal Year: 2024, Volume and Issue: 19(5), P. 052001 - 052001

Published: July 8, 2024

Abstract Wound healing is a critical but complex biological process of skin tissue repair and regeneration resulting from various systems working together at the cellular molecular levels. Quick wound problems associated with traditional techniques are being overcome multifunctional materials. Over time, this research area has drawn significant attention. Metal-organic frameworks (MOFs), owning to their peculiar physicochemical characteristics, now considered promising class well-suited porous materials for in addition other applications. This detailed literature review provides an overview latest developments MOFs We have discussed synthesis, essential biomedical properties, wound-healing mechanism, MOF-based dressing materials, The possible major challenges limitations been discussed, along conclusions future perspectives. addresses MOFs-based several angles covers most current subject. readers may discover how advanced discipline by producing more inventive, useful, successful dressings. It influences development generations biomaterials wounds.

Language: Английский

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Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Langmuir, Journal Year: 2022, Volume and Issue: 38(27), P. 8266 - 8279

Published: June 24, 2022

Peroxidase-like nanozymes with robust catalytic capacity and detection specificity have been proposed as substitutes to natural peroxidases in biochemical sensing. However, the activity enhancement, mechanism, application of nanozyme-based biosensors toward l-cysteine (l-Cys) still remain significant challenges. In this work, a doped ferrite nanozyme well-defined structure surface charges is fabricated by two-step method continuous flow coprecipitation high-temperature annealing. The resulted possesses an average size 54.5 nm zeta-potential 6.45 mV. A high-performance biosensor manufactured based on peroxidase-like feature ferrite. can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) assistance H2O2 because instinctive decompose into ·OH. Michaelis–Menten constants (0.0911 mM for TMB, 0.140 H2O2) are significantly smaller than those horseradish peroxidase. reliable colorimetric established selectively analyze l-Cys via facile mixing-and-detecting methodology. limit linear range 0.119 μM 0.2–20 μM, respectively. Taking merits biosensors, level human serum be qualitatively detected. It anticipated that surface-charged shows great prospects fields bioanalytical chemistry point-of-care testing.

Language: Английский

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Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

Biosensors, Journal Year: 2023, Volume and Issue: 13(9), P. 886 - 886

Published: Sept. 17, 2023

Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. The literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve stability sensitivity sensor. However, poor stability, weak reproducibility, limited lifetime itself still limit requirements development electrochemical food production process monitoring. Therefore, constructing sensing technologies remains a great challenge. This article outlines construction principles four generations discusses applications single-enzyme systems, multi-enzyme nano-enzyme systems developed these principles. further describes methods to immobilization by combining different types such as metals their oxides, graphene-related materials, metal-organic frameworks, carbon nanotubes, conducting polymers. In addition, highlights challenges future trends biosensors, providing theoretical support perspectives research high-performance chemical

Language: Английский

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Chemical amination of immobilized enzymes for enzyme coimmobilization: Reuse of the most stable immobilized and modified enzyme

International Journal of Biological Macromolecules, Journal Year: 2022, Volume and Issue: 208, P. 688 - 697

Published: March 28, 2022

Although Lecitase and the lipase from Thermomyces lanuginosus (TLL) could be coimmobilized on octyl-agarose, stability of was lower than that TLL causing user to discard active immobilized when inactivated. Here, we propose chemical amination ionically exchange TLL, which should released medium after its inactivation by incubation at high ionic strength. Using conditions where only adsorbed amination, combibiocatalyst produced. Unfortunately, release not possible using just strength solutions, if detergent added, also support. This occurred 0.25 M ammonium sulfate, did immobilize aminated TLL. That makes use octyl-vinylsulfone supports necessary irreversibly blocking with ethylendiamine, aminated. this biocatalyst sulfate 0.1% Triton X-100. way, a produced, inactivation, it immobilized, aminated, fully utilized build new combibiocatalyst.

Language: Английский

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Synthesis of l-aspartic acid-based bimetallic hybrid nanoflowers to immobilize snailase for the production of rare ginsenoside compound K

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 11(11), P. 2397 - 2408

Published: Jan. 1, 2023

1. Bimetallic hybrid nanoflowers containing l -aspartic acid were synthesized by a one-pot method for successful immobilizing snailase.2. Asp@ZIF-ZnCo-Sna has excellent enzyme loading capacity, activity and better catalytic efficiency.

Language: Английский

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Post-synthesis modification of metal–organic framework boosts solvent-free enzymatic esterifications

Journal of Catalysis, Journal Year: 2023, Volume and Issue: 421, P. 88 - 94

Published: March 5, 2023

Language: Английский

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Active CRISPR-Cas12a on Hydrophilic Metal–Organic Frameworks: A Nanobiocomposite with High Stability and Activity for Nucleic Acid Detection

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(28), P. 10580 - 10587

Published: July 6, 2023

CRISPR-Cas12a is an accurate and responsive biosensing technique, but its limited stability has restricted widespread applications. To address this, we propose a strategy using metal–organic frameworks (MOFs) to protect Cas12a from harsh environments. After screening multiple candidate MOFs, it was found that hydrophilic MAF-7 highly compatible with Cas12a, the as-formed Cas12a-on-MAF-7 (COM) not only retains high enzymatic activity also possesses excellent tolerance heat, salt, organic solvents. Further investigation showed COM can serve as analytical component for nucleic acid detection, resulting in ultrasensitive assay SARS-CoV-2 RNA detection limit of 1 copy. This first successful attempt create active nanobiocomposite functions biosensor without need shell deconstruction or enzyme release.

Language: Английский

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