Immobilization of Nitrilase: Driving Efficiency and Sustainability in Pharmaceutical Synthesis DOI
Rahul Vikram Singh

ChemBioEng Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract Enzyme immobilization has propelled the pharmaceutical sector forward by playing a critical role in chemical synthesis; therefore, biocatalyst reuse industries received significant attention due to its eco‐friendly nature and potential for streamlining future utilization. In particular, nitrilases have emerged as biocatalysts synthesizing intermediates active ingredients (APIs). created platform nitrilase that significantly improved thermal stability reusability, increasing commercial value industry. The current article provides comprehensive analysis of recent advances continuous flow systems, focusing on how this approach contributes efficiency sustainability exploitation advantages immobilization, such stability, ease isolation, along with biocatalysts, proven be an invaluable tool pursuit more environment‐friendly efficient synthesis.

Language: Английский

Global Advancements in Bioactive Material Manufacturing for Drug Delivery: A Comprehensive Study DOI Creative Commons
Rafael Leandro Fernandes Melo, Dayana Nascimento Dari, Francisco Izaias da Silva Aires

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(1), P. 1207 - 1225

Published: Jan. 3, 2025

Manufacturing bioactive materials for drug delivery involves developing that interact with biological tissues to release drugs in a controlled and targeted manner. The goal is optimize therapeutic efficacy reduce side effects by combining knowledge from engineering, biology, pharmacology. This study presents detailed bibliometric analysis, exploring the keywords "manufacturing," "bioactive materials," "drug delivery" identify highlight significant advancements field. From Web of Science, 36,504 articles were analyzed, 171 selected deeper identifying key journals, countries, institutions, authors. results field's interdisciplinary nature, grouped into four main themes, including regenerative medicine, scaffolds, three-dimensional (3D) printing, glass, tissue engineering. Future research this area will focus on more effective precise systems using technologies like 3D printing nanotechnology enhance customization control release, aiming efficient therapies.

Language: Английский

Citations

4

Advancements and Prospects in Nanorobotic Applications for Ophthalmic Therapy DOI Creative Commons
Achilles Nina Santos Ferreira, John Hebert da Silva Félix, Rita Karolinny Chaves de Lima

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

This study provides a bibliometric and bibliographic review of emerging applications micro- nanotechnology in treating ocular diseases, with primary focus on glaucoma. We aim to identify key research trends analyze advancements devices drug delivery systems for treatments. The methodology involved analyzing 385 documents indexed the Web Science using tools such as VOSviewer Bibliometrix. results show marked increase scientific output, highlighting prominent authors institutions, England leading field. Key findings suggest that holds potential address limitations conventional treatments, including low bioavailability adverse side effects. Nanoparticles, nanovesicles, polymer-based appear promising prolonged controlled release, potentially offering enhanced therapeutic efficacy. In conclusion, could transform disease treatment, although challenges remain concerning biocompatibility scalability these devices. Further clinical studies are necessary establish innovations within context ophthalmology.

Language: Английский

Citations

3

The current research status of immobilized lipase performance and its potential for application in food are developing toward green and healthy direction: A review DOI
Ning Wang,

Weizhe Wang,

Yufeng Su

et al.

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(2)

Published: Feb. 1, 2025

Abstract Immobilized lipases have received great attention in food, environment, medicine, and other fields due to their easy separation, high stability (temperature, pH), storage properties. After immobilization, lipase transforms from a homogeneous heterogeneous state, making it easier recover the reaction substrate achieve recycling, which is line with concept of green chemistry reduces protein contamination product. There are various materials for enzyme including polysaccharides natural sources, inorganic compounds, carbon nanotubes, metal–organic framework materials, so forth. Magnetic immobilization carriers been widely studied ability separation by adding magnetic field. Its method can be simply divided into two categories: physical action (adsorption, embedding) chemical binding (covalent, cross‐linking). Some studies mainly discuss support methods, applications immobilized food. On this basis, our review also focuses on changes crosslinking agents lipases, different methods promote new trends study proposes prospects research food industry.

Language: Английский

Citations

0

Advances and Applications of Micro- and Mesofluidic Systems DOI Creative Commons
Luís Alves, John Hebert da Silva Félix, Achilles Nina Santos Ferreira

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Microfabrication technology has advanced scientific understanding and expanded our molecular control capabilities, enabling the development of 3D models in micrometer structures. The sizes fluidic channels are arranged descending order, starting with macro-, followed by meso-, micro-, nanoscale. These advances bring advantages speed up biological chemical experimental processes. Such miniaturized systems show significant advances, particularly meso- microreactors, through high-throughput screening. This work proposes a bibliometric analysis applications Web Science (WoS) database, analyzing main highlights publications, indicators, impact on knowledge production. In past 20 years, approximately 3,934 documents published cited, mainly major world powers micro- mesofluidic systems, increasingly expanding academic industrial sectors.

Language: Английский

Citations

0

Heterologous expression and enzymatic properties of lipase from Mucor circinelloides DOI Creative Commons
Yao Zhang, Yan Sun, Zhuo Liu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 29, 2025

Microbial lipases could be used to hydrolyze or recombine fats and oils, had great applications in food processing, bioenergy chemical industry. Mucor circinelloides was an important gamma-linolenic acid producing strain, its genome predicted contain a large number of genes encoding lipases, the key enzymes lipid metabolism. In present study, potential lipase WJ_23 from WJ11 cloned for first time heterologously expressed purified homogeneity Pichia pastoris. By SDS-PAGE analysis, molecular weight recombinant estimated ~ 34 kDa. The optimal temperature pH were 50 °C 9.0, respectively. good thermal stability at with broad 6.0 11.0. After incubation 37 24 h, activity remained over 95% between 7.0 9.0. possessed preference long chain substrates. Using p-NPP as substrate, measured kinetic parameters Vmax Km 94.34 U/mg 6.37 mmoL/L, addition, not affected obviously by various metal ions, it exhibited certain tolerance towards several kinds organic solvents. This research might provide basis further industrial application M. lipase.

Language: Английский

Citations

0

Immobilization of Nitrilase: Driving Efficiency and Sustainability in Pharmaceutical Synthesis DOI
Rahul Vikram Singh

ChemBioEng Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract Enzyme immobilization has propelled the pharmaceutical sector forward by playing a critical role in chemical synthesis; therefore, biocatalyst reuse industries received significant attention due to its eco‐friendly nature and potential for streamlining future utilization. In particular, nitrilases have emerged as biocatalysts synthesizing intermediates active ingredients (APIs). created platform nitrilase that significantly improved thermal stability reusability, increasing commercial value industry. The current article provides comprehensive analysis of recent advances continuous flow systems, focusing on how this approach contributes efficiency sustainability exploitation advantages immobilization, such stability, ease isolation, along with biocatalysts, proven be an invaluable tool pursuit more environment‐friendly efficient synthesis.

Language: Английский

Citations

0