Nanomolar PFOA Concentrations Affect Lipid Membrane Structure: Consequences for Bioconcentration Mechanisms DOI

Tess N. Sobolewski,

Rhys C. Trousdale,

Colin Gauvin

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

Independent methods show that sub-microMolar concentrations of perfluorooctanoic acid (PFOA), a member the PFAS family "forever chemicals", change properties DPPC vesicle bilayers. Specifically, calorimetry measurements PFOA at as low 0.1 nM lowers DPPC's gel–liquid crystalline transition enthalpy by several J/g without changing temperature (Tgel-LC), and dynamic light scattering (DLS) data illustrate markedly broadens size distribution vesicles. Furthermore, DLS results from PFOA-containing, solutions also contain smaller objects having diameters 30–50 nm. Close inspection cryo-EM images reveals vesicles formed in presence are multilamellar have clear bilayer structure similar to niosomes. A consequence these PFOA-induced changes is bilayers themselves more susceptible secondary solute accumulation. Time resolved emission Coumarin 152 (C152) report C152 3-fold likely partition into bilayer's acyl chain, hydrophobic interior when present, fluorescence lifetimes partitioned polar region lipid evidence membrane hydration below Tgel-LC.

Language: Английский

Nanomolar PFOA Concentrations Affect Lipid Membrane Structure: Consequences for Bioconcentration Mechanisms DOI

Tess N. Sobolewski,

Rhys C. Trousdale,

Colin Gauvin

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

Independent methods show that sub-microMolar concentrations of perfluorooctanoic acid (PFOA), a member the PFAS family "forever chemicals", change properties DPPC vesicle bilayers. Specifically, calorimetry measurements PFOA at as low 0.1 nM lowers DPPC's gel–liquid crystalline transition enthalpy by several J/g without changing temperature (Tgel-LC), and dynamic light scattering (DLS) data illustrate markedly broadens size distribution vesicles. Furthermore, DLS results from PFOA-containing, solutions also contain smaller objects having diameters 30–50 nm. Close inspection cryo-EM images reveals vesicles formed in presence are multilamellar have clear bilayer structure similar to niosomes. A consequence these PFOA-induced changes is bilayers themselves more susceptible secondary solute accumulation. Time resolved emission Coumarin 152 (C152) report C152 3-fold likely partition into bilayer's acyl chain, hydrophobic interior when present, fluorescence lifetimes partitioned polar region lipid evidence membrane hydration below Tgel-LC.

Language: Английский

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