Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances DOI Creative Commons

Yiwen Wang,

Hong-Fei Tong,

Shulan Ni

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Immobilizing enzymes onto solid supports having enhanced catalytic activity and resistance to harsh external conditions is considered as a promising critical method of broadening enzymatic applications in biosensing, biocatalysis, biomedical devices; however, it considerably hampered by limited strategies. Here, core-shell strategy involving soft-core hexahistidine metal assembly (HmA) innovatively developed characterized with encapsulated (catalase (CAT), horseradish peroxidase, glucose oxidase (GOx), cascade (CAT+GOx)) hard porous shells (zeolitic imidazolate framework (ZIF), ZIF-8, ZIF-67, ZIF-90, calcium carbonate, hydroxyapatite). The enzyme-friendly environment provided the embedded HmA proves beneficial for activity, which particularly effective preserving fragile that will have been deactivated without core during mineralization shells. enzyme within particle exhibits noteworthy resilience against conditions, including heat, organic solvents, proteinase K. Additionally, no significant alteration behavior observed after multiple cycles usage. This study offers novel approach immobilizing rendering them resistant potential diverse fields, bioremediation, engineering.

Language: Английский

Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances DOI Creative Commons

Yiwen Wang,

Hong-Fei Tong,

Shulan Ni

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Immobilizing enzymes onto solid supports having enhanced catalytic activity and resistance to harsh external conditions is considered as a promising critical method of broadening enzymatic applications in biosensing, biocatalysis, biomedical devices; however, it considerably hampered by limited strategies. Here, core-shell strategy involving soft-core hexahistidine metal assembly (HmA) innovatively developed characterized with encapsulated (catalase (CAT), horseradish peroxidase, glucose oxidase (GOx), cascade (CAT+GOx)) hard porous shells (zeolitic imidazolate framework (ZIF), ZIF-8, ZIF-67, ZIF-90, calcium carbonate, hydroxyapatite). The enzyme-friendly environment provided the embedded HmA proves beneficial for activity, which particularly effective preserving fragile that will have been deactivated without core during mineralization shells. enzyme within particle exhibits noteworthy resilience against conditions, including heat, organic solvents, proteinase K. Additionally, no significant alteration behavior observed after multiple cycles usage. This study offers novel approach immobilizing rendering them resistant potential diverse fields, bioremediation, engineering.

Language: Английский

Citations

0