Side-Functionalization of Poly(l-methionine) for Ice Control DOI

Qingjing Niu,

Ke Shang,

Huimin Han

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Controlling ice growth is crucial during the cryopreservation of cells, but current application small molecules as cryoprotectants still remains a challenge. Inspired by structures natural antifreeze (glyco)proteins, in this work, functionalized poly(l-methionine)s (PMets) are synthesized with different side groups including hydroxyl, threonine-mimetic both methyl and hydroxyl (PMet-MOH), zwitterion carboxyl sulfonium (PMet-COOH), glycerol, trehalose pendants. Results suggest that these PMets tend to self-assemble into 100-300 nm nanoparticles positive charges water. The functional have remarkable influence on their control properties. It supposed PMet-MOH inhibits possibly through adsorption mechanism adjacent groups, whereas trehalose-tethered PMet can restrict diffusion water strongest recrystallization inhibition activity zwitterionic PMet-COOH promotes nucleation obviously. This work offers valuable insight development polypeptides promising biocompatible cryoprotectants.

Language: Английский

Side-Functionalization of Poly(l-methionine) for Ice Control DOI

Qingjing Niu,

Ke Shang,

Huimin Han

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Controlling ice growth is crucial during the cryopreservation of cells, but current application small molecules as cryoprotectants still remains a challenge. Inspired by structures natural antifreeze (glyco)proteins, in this work, functionalized poly(l-methionine)s (PMets) are synthesized with different side groups including hydroxyl, threonine-mimetic both methyl and hydroxyl (PMet-MOH), zwitterion carboxyl sulfonium (PMet-COOH), glycerol, trehalose pendants. Results suggest that these PMets tend to self-assemble into 100-300 nm nanoparticles positive charges water. The functional have remarkable influence on their control properties. It supposed PMet-MOH inhibits possibly through adsorption mechanism adjacent groups, whereas trehalose-tethered PMet can restrict diffusion water strongest recrystallization inhibition activity zwitterionic PMet-COOH promotes nucleation obviously. This work offers valuable insight development polypeptides promising biocompatible cryoprotectants.

Language: Английский

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