Layer-by-layer coating strategy to functionalize the magnetic nanoparticles for their multi-functionalization DOI Creative Commons
Jing Liu,

Ye Chen,

Hongjie Huang

et al.

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: May 2, 2025

Abstract Magnetic nanoparticles (MNPs) hold significant potential for a wide range of applications, however, surface modification or bio-conjugation MNPs often leads to their aggregation and instability. To address this, we proposed facile method using layer-by-layer (LbL) coating technique with polyallylamine hydrochloride (PAH) poly(styrene sulfonic acid) sodium salt (PSS), so as maintain the dispersion stability functionality MNPs. This enabled us develop powerful towards use in electrochemical biosensor, by combining both redox probes (ferrocene (Fc), anthraquinone (AQ), monocarboxymethylene blue (MB)) bio-probes (IgG). The molecules were effectively anchored under organic solvents, while such functionalized subsequently protected LbL process prior dispersing high ionic strength solutions (e.g. Phosphate-buffered saline). And out-layer polyelectrolyte shell allowed biomolecules attach MNP without chemical cross-linking. Our results demonstrated that TEM size MNPs@Fc, MNP@AQ MNP@MB after characterized 11.0 ± 2.0 nm, 10.5 2.1 nm 12.4 2.2 these developed MNPs@AQ MNPs@MB square wave voltammetry (SWV) intensity 0.64 0.10 µA, 23.25 0.73 µA 0.48 0.13 respectively. In addition, binding efficiency adsorption between IgG was up 78%, evidenced SDS-PAGE gel analysis. offered versatile effective way functionalize MNPs, biological properties applications disease diagnosis point-of-care diagnostics.

Language: Английский

Layer-by-layer coating strategy to functionalize the magnetic nanoparticles for their multi-functionalization DOI Creative Commons
Jing Liu,

Ye Chen,

Hongjie Huang

et al.

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: May 2, 2025

Abstract Magnetic nanoparticles (MNPs) hold significant potential for a wide range of applications, however, surface modification or bio-conjugation MNPs often leads to their aggregation and instability. To address this, we proposed facile method using layer-by-layer (LbL) coating technique with polyallylamine hydrochloride (PAH) poly(styrene sulfonic acid) sodium salt (PSS), so as maintain the dispersion stability functionality MNPs. This enabled us develop powerful towards use in electrochemical biosensor, by combining both redox probes (ferrocene (Fc), anthraquinone (AQ), monocarboxymethylene blue (MB)) bio-probes (IgG). The molecules were effectively anchored under organic solvents, while such functionalized subsequently protected LbL process prior dispersing high ionic strength solutions (e.g. Phosphate-buffered saline). And out-layer polyelectrolyte shell allowed biomolecules attach MNP without chemical cross-linking. Our results demonstrated that TEM size MNPs@Fc, MNP@AQ MNP@MB after characterized 11.0 ± 2.0 nm, 10.5 2.1 nm 12.4 2.2 these developed MNPs@AQ MNPs@MB square wave voltammetry (SWV) intensity 0.64 0.10 µA, 23.25 0.73 µA 0.48 0.13 respectively. In addition, binding efficiency adsorption between IgG was up 78%, evidenced SDS-PAGE gel analysis. offered versatile effective way functionalize MNPs, biological properties applications disease diagnosis point-of-care diagnostics.

Language: Английский

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