Aqueous Synthesis of Poly(ethylene glycol)‐Amide‐Norbornene‐Carboxylate for Modular Hydrogel Crosslinking

Advanced Materials Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Abstract Poly(ethylene glycol)‐norbornene (e.g., PEGNB) is a versatile macromer amenable to step‐growth thiol‐norbornene photopolymerization and inverse electron demand Diels–Alder (iEDDA) click reaction. The translational potentials of PEGNB‐based hydrogels have been realized in the areas stem cell differentiation, vitro disease modeling, implantable therapeutic devices, controlled release therapeutics. Even with these advances, prior methods for synthesizing PEGNB all required heavy use organic solvents that pose significant environmental personal health burdens. Here, an all‐aqueous synthesis PEG‐amide‐norbornene‐carboxylate (PEGaNB CA ) reported via reacting carbic anhydride (CA) multi‐arm amino‐terminated PEG. Like previously ester‐bearing counterparts (i.e., PEGeNB ), PEGaNB readily crosslinked into modular by either or tetrazine‐norbornene iEDDA Unlike its counterparts, provided long‐term hydrolytic stability. However, through blending hydrolytically labile , can be engineered undergo tunable degradation. versatility further demonstrated high‐fidelity digital light processing printing situ encapsulation maintenance human induced pluripotent cells (hiPSCs).

Language: Английский

---

Methods to achieve tissue-mimetic physicochemical properties in hydrogels for regenerative medicine and tissue engineering

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(35), P. 8505 - 8522

Published: Jan. 1, 2024

Hydrogels are limited by their inability to recapitulate native extracellular physicochemical properties. Current efforts using advanced materials science and engineering methods seek form hydrogels with physiologically-relevant

Language: Английский

---

Impact of Annealing Chemistry on the Properties and Performance of Microporous Annealed Particle Hydrogels

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(9), P. 5798 - 5808

Published: Aug. 27, 2024

Microporous annealed particle (MAP) hydrogels are a promising class of in situ-forming scaffolds for tissue repair and regeneration. While an expansive toolkit annealing chemistries has been described, the effects different on MAP hydrogel properties performance have not studied. In this study, we address gap through controlled head-to-head comparison poly(ethylene glycol) (PEG)-based that were using tetrazine-norbornene thiol-norbornene click chemistry. Characterization material revealed tetrazine significantly increases shear storage modulus results slower vitro degradation kinetics when microgels with higher cross-link density used. However, these muted fabricated from lower density. contrast, vivo testing murine critical-sized calvarial defects differences physicochemical do translate to bone volume or defect healing growth-factor-loaded implanted into mouse defects. Nonetheless, impact could be important other applications should investigated further.

Language: Английский

---

Injectable long-acting formulations (ILAFs) and manufacturing techniques

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: 21(6), P. 881 - 904

Published: June 2, 2024

Most therapeutics delivered using short-acting formulations need repeated administration, which can harm patient compliance and raise failure risks related to inconsistent treatment. Injectable long-acting (ILAFs) are controlled/sustained-release fabricated deliver active pharmaceutical ingredients (APIs) extend their half-life over days months. Longer half-lives of ILAFs minimize the necessity for frequent doses, increase compliance, reduce risk side effects from intravenous (IV) infusions. Using ILAF technologies, immediate drug release also be controlled, thereby minimizing potential adverse due high initial blood concentrations.

Language: Английский

---