Facially Amphiphilic Cholate-Conjugated Polymers for Regulating Insulin Fibrillation DOI
Desoshree Ghosh, Sagar Bag, Priyadarsi De

et al.

Bioconjugate Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

To understand the influence of facially amphiphilic polymers (FAPs) on insulin fibril (IF) inhibition, three different cholate-based FAPs [cationic (PFCAQA), anionic (PFCASF), and zwitterionic (PFCASB)] have been synthesized. Besides, two control [cholate sulfobetaine-pendant random copolymer PRCASB (without facial amphiphilicity) sulfobetaine-tethered homopolymer PSBMA cholate pendants)] are also prepared. Several biophysical experiments such as spectroscopic techniques [thioflavin T (ThT), Nile red (NR), tyrosine (Tyr) fluorescence assay], turbidity assay by ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), circular dichroism (CD) study, microscopic investigation performed to investigate role antiamyloidogenic agents during fibrillation. Interestingly, PFCASB polymer behaves most efficacious agent. clarify interaction native (NI), an isothermal titration calorimetry (ITC) experiment is carried out. Tyr NR suggest important hydrophobic interactions, whereas ITC confirms significance electrostatic interactions in IF inhibitory process. A hemolytic test conducted toxicity caused efficacy prohibiting erythrocyte disruption IF. Overall, present work reveals impact cholic acid (CA)-based modulating aggregation process gives a new perspective for investigations protein aggregations.

Language: Английский

Facially Amphiphilic Cholate-Conjugated Polymers for Regulating Insulin Fibrillation DOI
Desoshree Ghosh, Sagar Bag, Priyadarsi De

et al.

Bioconjugate Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

To understand the influence of facially amphiphilic polymers (FAPs) on insulin fibril (IF) inhibition, three different cholate-based FAPs [cationic (PFCAQA), anionic (PFCASF), and zwitterionic (PFCASB)] have been synthesized. Besides, two control [cholate sulfobetaine-pendant random copolymer PRCASB (without facial amphiphilicity) sulfobetaine-tethered homopolymer PSBMA cholate pendants)] are also prepared. Several biophysical experiments such as spectroscopic techniques [thioflavin T (ThT), Nile red (NR), tyrosine (Tyr) fluorescence assay], turbidity assay by ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), circular dichroism (CD) study, microscopic investigation performed to investigate role antiamyloidogenic agents during fibrillation. Interestingly, PFCASB polymer behaves most efficacious agent. clarify interaction native (NI), an isothermal titration calorimetry (ITC) experiment is carried out. Tyr NR suggest important hydrophobic interactions, whereas ITC confirms significance electrostatic interactions in IF inhibitory process. A hemolytic test conducted toxicity caused efficacy prohibiting erythrocyte disruption IF. Overall, present work reveals impact cholic acid (CA)-based modulating aggregation process gives a new perspective for investigations protein aggregations.

Language: Английский

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