Revolutionizing mRNA Vaccines Through Innovative Formulation and Delivery Strategies

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 359 - 359

Published: March 1, 2025

Modernization of existing methods for the delivery mRNA is vital in advanced therapeutics. Traditionally, has faced obstacles poor stability due to enzymatic degradation. This work examines cutting-edge formulation and emerging techniques safer vaccines. Inspired by success lipid nanoparticles (LNP) delivering vaccines COVID-19, a variety other formulations have been developed deliver diverse infections. The meritorious features nanoparticle-based strategies, including LNP, polymeric, dendrimers, polysaccharide-based, peptide-derived, carbon metal-based, DNA nanostructures, hybrid, extracellular vesicles, examined. impact these platforms on vaccine efficacy, protection from degradation, cellular uptake, controlled release, immunogenicity discussed detail. Even with significant developments, there are certain limitations overcome, toxicity concerns, limited information about immune pathways, need maintain cold chain, necessity optimizing administration methods. Continuous innovation essential improving systems Future research directions proposed address challenges expand their potential prophylactic therapeutic application.

Language: Английский

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Enhancing Vaccine Efficacy and Stability: A Review of the Utilization of Nanoparticles in mRNA Vaccines

Biomolecules, Journal Year: 2024, Volume and Issue: 14(8), P. 1036 - 1036

Published: Aug. 20, 2024

The development of vaccines has entered a new era with the advent nanotechnology, particularly through utilization nanoparticles. This review focuses on role nanoparticles in enhancing efficacy and stability mRNA vaccines. Nanoparticles, owing to their unique properties such as high surface area, tunable size, ability be functionalized, have emerged powerful tools vaccine development. Specifically, lipid (LNPs) revolutionized delivery by protecting fragile molecules facilitating efficient uptake cells. discusses various types employed formulations, including lipid-based, polymer-based, inorganic nanoparticles, highlighting advantages limitations. Moreover, it explores mechanisms which improve immune responses, enhanced antigen presentation prolonged release mRNA. also addresses challenges future directions nanoparticle-based development, emphasizing need for further research optimize formulations broader applications. By providing an in-depth analysis current advancements potential vaccines, this aims shed light critical combating infectious diseases improving public health outcomes.

Language: Английский

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Improving the use of CRISPR/Cas9 gene editing machinery as a cancer therapeutic tool with the help of nanomedicine

3 Biotech, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 19, 2024

Language: Английский

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State of the art in CAR-based therapy: In vivo CAR production as a revolution in cell-based cancer treatment

Cellular Oncology, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Chimeric antigen receptor (CAR) therapy has successfully treated relapsed/refractory hematological cancers. This strategy can effectively target tumor cells. However, despite positive outcomes in clinical applications, challenges remain to overcome. These hurdles pertain the production of drugs, solid resistance, and side effects related treatment. Some cases have been missed during drug preparation due manufacturing issues, prolonged times, high costs. mainly arise from vitro process, so reevaluating this process could minimize number patients. The immune cells are traditionally collected sent laboratory; after several steps, modified express CAR gene before being injected back into patient's body. During vivo method, is introduced inside allows for treatment begin sooner, avoiding potential failures associated In review, we will elaborate on using CAR, examine benefits approach, ultimately present available solutions incorporating practice.

Language: Английский

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Transition Temperature-Guided Design of Lipid Nanoparticles for Effective mRNA Delivery

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Lipid nanoparticles (LNPs) are promising mRNA delivery vehicles due to their biocompatibility and tunable characteristics. While current rational design approaches focus on ionizable lipids' pKa zeta potential optimize encapsulation endosomal escape, the selection of helper lipids remains largely empirical. We propose that lipid transition temperature (Tm), marking shift from gel liquid crystalline phase, can guide selection. Through screening 54 lipids, we identified H7T4, which displayed favorable physicochemical properties when combined with its tail variants but exhibited poor transfection efficiency. Using nano differential scanning calorimetry (nDSC) biological small-angle X-ray scattering (BioSAXS), found lowering system's Tm by combining H7T4 (high temperature) a low-transition-temperature such as 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) significantly enhanced cellular uptake both in vitro vivo. These findings establish crucial parameter for LNP design.

Language: Английский

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Advances in COVID-19 and Cancer Research

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(14), P. 4143 - 4143

Published: July 16, 2024

The global health crisis caused by COVID-19 has radically changed the management of several diseases [...]

Language: Английский

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mRNA-based cancer vaccines: A novel approach to melanoma treatment

Advances in immunology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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