Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(12), P. 3470 - 3488

Published: Aug. 15, 2024

Language: Английский

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Advances in design and preparation of nanozymes and their applications for constructing higher sensitive lateral flow assays

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 510, P. 215797 - 215797

Published: April 4, 2024

Language: Английский

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Nanozyme‐Enabled Biomedical Diagnosis: Advances, Trends, and Challenges

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 13, 2024

Abstract As nanoscale materials with the function of catalyzing substrates through enzymatic kinetics, nanozymes are regarded as potential alternatives to natural enzymes. Compared protein‐based enzymes, exhibit attractive characteristics low preparation cost, robust activity, flexible performance adjustment, and versatile functionalization. These advantages endow them wide use from biochemical sensing environmental remediation medical theranostics. Especially in biomedical diagnosis, feature catalytic signal amplification provided by makes emerging labels for detection biomarkers diseases, rapid developments observed recent years. To provide a comprehensive overview progress made this dynamic field, here an diagnosis enabled is provided. This review first summarizes synthesis nanozyme then discusses main strategies applied enhance their activity specificity. Subsequently, representative utilization combined biological elements disease reviewed, including related metabolic, cardiovascular, nervous, digestive diseases well cancers. Finally, some development trends nanozyme‐enabled highlighted, corresponding challenges also pointed out, aiming inspire future efforts further advance promising field.

Language: Английский

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Color- and background-free Raman-encoded lateral flow immunoassay for simultaneous detection of carbendazim and imidacloprid in a single test line

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 487, P. 150666 - 150666

Published: March 24, 2024

Language: Английский

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“Three-in-One” Plasmonic Au@PtOs Nanocluster Driven Lateral Flow Assay for Multimodal Cancer Exosome Biosensing

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10686 - 10695

Published: June 17, 2024

Exploiting the multiple properties of nanozymes for multimode lateral flow assay (LFA) is urgently required to improve accuracy and versatility. Herein, we developed a novel plasmonic Au nanostar@PtOs nanocluster (Au@PtOs) as signal tag LFA detection. Based on PtOs bimetallic doping strategy, Au@PtOs can indicate both excellent SERS enhancement nanozyme catalytic activity. Meanwhile, displays better photothermal effect than that nanostars. Therefore, colorimetric/SERS/temperature three-mode signals be read out based nanocomposite. The was combined with applied breast cancer exosome detection limit mode 2.6 × 103/4.1 101/4.6 102 exosomes/μL, respectively, which much superior common nanoparticles (∼105 exosomes/μL). Moreover, fingerprint molecular recognition ability mode, phenotypes derived from different cell lines discriminated easily. convenient visual colorimetric sensitive SERS/temperature quantitative modes, driven satisfy requirements accurate flexible multimodal sensing in application scenarios.

Language: Английский

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3D Film-Like Nanozyme with a Synergistic Amplification Effect for the Ultrasensitive Immunochromatographic Detection of Respiratory Viruses

ACS Nano, Journal Year: 2024, Volume and Issue: 18(37), P. 25865 - 25879

Published: Sept. 2, 2024

Greatly improving the sensitivity and detection range of lateral flow immunoassays (LFAs) by at least 100 times without using additional instruments remains challenging. Herein, we develop a three-dimensional (3D) film-like nanozyme (GO-Pt

Language: Английский

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A Wash-Free Spheres-on-Sphere Strategy for On-Site and Multiplexed Biosensing

ACS Nano, Journal Year: 2024, Volume and Issue: 18(11), P. 8270 - 8282

Published: March 7, 2024

Respiratory infections and food contaminants pose severe challenges to global health the economy. A rapid on-site platform for simultaneous detection of multiple pathogens is crucial accurate diagnosis, appropriate treatment, a reduced healthcare burden. Herein, we present spheres-on-sphere (SOS) multiplexed using portable Coulter counter, which employs millimeter- micron-sized spheres coupled with antibodies as multitarget probes. The assay allows quantitative analytes within 20 min by simple mixing, enabling detection. shows high accuracy in identifying three respiratory viruses (SARS-CoV-2, influenza virus, parainfluenza virus) from throat swab samples, LOD 50.7, 32.4, 49.1 pg/mL. It also demonstrates excellent performance quantifying mycotoxins (aflatoxin B1, deoxynivalenol, ochratoxin A) samples. SOS offers approach sensitivity specificity applications resource-limited settings.

Language: Английский

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Bimetallic Nanozymes‐Integrated Parachute‐Like Au₂Pt@PMO@ICG Janus Nanomotor with Dual Propulsion for Enhanced Tumor Penetration and Synergistic PTT/PDT/CDT Cancer Therapy

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(44)

Published: Aug. 12, 2024

Abstract Nanocatalytic therapeutic agents with triple synergistic treatment modes of chemodynamic therapy (CDT), photothermal (PTT) and photodynamic (PDT) are emerging nanomaterials in malignancy treatment. Nevertheless, the passive diffusion transport result poor permeability tumor lesions, severely affecting effectiveness therapy. Herein, a dual‐source‐driven parachute‐like Au 2 Pt@PMO@ICG Janus nanomotor (APIJNS) is prepared by an interfacial energy‐mediated anisotropic growth strategy for PTT‐mediated CDT/PDT cancer Such can realize self‐thermophoresis drive under trigger near‐infrared light, which effectively enhance active uptake APIJNS tissue, thus achieving efficient PTT/PDT/CDT effect. In addition, exhibits high‐efficiency catalase (CAT)‐like activity catalyze overexpressed H O microenvironment (TME) to generate oxygen (O ), not only alleviating hypoxia lesion, but also converting it into singlet ( 1 ) activate photosensitizer ICG, PDT. This work provides new ideas enhancing improving dual‐drive nanomotors based on dual noble metal nanozymes.

Language: Английский

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Metal Ion-Based Chemical Coordination Amplification: A Chromophore In Situ Deposition Strategy for Visual Sensitivity-Enhanced Lateral Flow Immunochromatography Assays

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Traditional lateral flow immunochromatography assays (LFIAs) have faced low sensitivity for trace detection due to the lack of colorimetric brightness. The current strategies improve commonly disadvantages an uncontrollable enhancement process or high background interference, leading huge obstacles signal readout. Herein, in situ metal ion-based chemical coordination amplification (MICCA) strategy has been reported. Metal ion clusters on metal–organic frameworks could coordinate with chromophores produce colored complexes visual enhancement. A Zr-based AIEgen framework (MAF) loaded Prussian blue was chosen as dual-mode tag and fluorescent MAF be employed a grafting substrate deposit through Zr4+ arsenazo III. MICCA situ, controllable, free interference. For target cancer biomarker alpha-fetoprotein (AFP), limit (LOD) by naked eye 25 ng/mL, LODs fluorescence were 5 which 5-fold decreased. Significantly, MICCA-LFIA effectively differentiate between AFP-positive AFP-negative clinical serum samples. quantitative results highly consistent (R2 = 0.9927). This work explored application reactions provided ideas high-sensitivity LFIA development.

Language: Английский

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Advances in total antioxidant capacity detection based on nanozyme

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 127941 - 127941

Published: March 13, 2025

Language: Английский

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