Published: Jan. 1, 2024
Language: Английский
Science Advances, Journal Year: 2024, Volume and Issue: 10(45)
Published: Nov. 6, 2024
Biological nanopores are increasingly used in molecular sensing due to their single-molecule sensitivity. The detection of per- and polyfluoroalkyl substances (PFAS) like perfluorooctanoic acid perfluorooctane sulfonic is critical environmental prevalence toxicity. Here, we investigate selective interactions between PFAS four cyclodextrin (CD) variants (α-, β-, γ-, 2-hydroxypropyl-γ-CD) within an α-hemolysin nanopore. We demonstrate that molecules can be electrochemically sensed by interacting with a γ-CD Using HP-γ-CDs increased steric resistance, identify homologs the perfluoroalkyl carboxylic families detect common drinking water at 0.4 2 parts per million levels, which further lowered 400 trillion sample preconcentration. Molecular dynamics simulations reveal underlying chemical mechanism PFAS-CD interactions. These insights pave way toward nanopore-based situ promises protection against pollution.
Language: Английский
Citations
4
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137407 - 137407
Published: Feb. 1, 2025
Language: Английский
Citations
0
Expert Review of Proteomics, Journal Year: 2025, Volume and Issue: unknown
Published: March 19, 2025
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been a leading method for proteomics thirty years. Advantages provided by LC-MS/MS are offset significant disadvantages, including cost. Recently, several non-mass spectrometric methods have emerged, but little information is available about their capacity to analyze the complex mixtures routine spectrometry. We review recent non-mass-spectrometric sequencing proteins and peptides, using nanopores, degradation, reverse translation, short-epitope mapping, with comments on bioinformatics challenges, fundamental limitations, areas where new technologies will be more or less competitive LC-MS/MS. In addition conventional literature searches, instrument vendor websites, patents, webinars, preprints were also consulted give up-to-date picture. Many promising. However, demonstrations that they outperform in terms of peptides identified not yet published, astute observers note important especially relating dynamic range single-molecule measurements mixtures. Still, even if performance emerging proves inferior LC-MS/MS, low cost could create different kind revolution: dramatic increase number biology laboratories engaging forms research.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 16, 2025
Multiplexed and portable detection of small molecular food contaminants in complex matrices remains a challenge analytical chemistry. Herein, inspired by the multiplexed detection, nucleic-acid-targeting capability, programmability Thermus thermophilus argonaute (TtAgo), fingerprint recognition nanopore technology, we proposed TtAgo-assisted encoding strategy to detect three mycotoxins simultaneously (AFB1, OTA, ZEN). In this method, effective signal conversion turns into DNA reporters that were observed via technology . general, guide DNAs (gDNAs) released presence mycotoxins, followed specific cleavage mediated TtAgo release reporters. The translocation produces highly distinguishable information due favorable resolution nanopore. Owing high nanopore, method displayed low LODs (AFB1 67.18 fM, OTA 13.32 ZEN 11.69 fM) with wide linear ranges. Furthermore, specificity practical application also verified showed satisfactory results. conclusion, offers novel avenue for molecules.
Language: Английский
Citations
0
Talanta, Journal Year: 2024, Volume and Issue: 283, P. 127136 - 127136
Published: Nov. 1, 2024
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0