The potential of carbohydrate supramolecular hydrogels for long-term 3D culture of primary fibroblasts

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

A pure small molecule, N -nonyl- d -galactonamide self-assembles into supramolecular fibers to give hydrogels. The cell growth and morphology, on the top inside of these fibrous hydrogels, were analyzed over several weeks.

Language: Английский

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Cellulose-mediated mechanical property tuning in small intestinal submucosal matrix to enhance stem cell osteogenic differentiation

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139575 - 139575

Published: Jan. 8, 2025

Language: Английский

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Synthesis, characterization, and biological evaluation of “Bamboo lamellar" biomimetic hydroxyapatite

Ceramics International, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Effect of Fluorophilic- and Hydrophobic-Modified Polyglycerol-Based Coatings on the Wettability of Low Surface Energy Polymers

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Catechol-derived polymers form stable coatings on a wide range of materials including challenging to coat low surface energy polymers. Whether modification the coating polymer with fluorophilic or hydrophobic groups is successful approach further favor surfaces catechol-based remains ambiguous. Herein, we report effect series catechol-derived polyglycerol (PG)-based and monolayer wettability polytetrafluoroethylene (PTFE), polystyrene, poly(methyl methacrylate) surfaces. Coatings longer hydrophilic PG block resulted in water contact angles (WCAs) around 60° independently substrate, while anchoring possessed more diverse WCAs up (129 ± 10)°. Despite generally small impact for most substrate/coating combinations, some modified reduce WCA PTFE below Berg's limit 65°, indicating shielding fluorous segments from surface.

Language: Английский

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Recent advances of physiochemical cues on surfaces for directing cell fates

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 250, P. 114550 - 114550

Published: Feb. 6, 2025

Language: Английский

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Label-Free Optical Mapping for Large-Area Biomechanical Dynamics of Multicellular Systems

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 277, P. 117281 - 117281

Published: Feb. 18, 2025

Language: Английский

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Efficient Differentiation of hiPSCs into hMSC-like Cells under Chemically Defined Conditions on Temperature-Sensitive Micropatterned Surfaces

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: 17(9), P. 13358 - 13374

Published: Feb. 20, 2025

The fairness of long-term self-renewal and robust cell proliferation limits the applications human mesenchymal stem cells (hMSCs) in regenerative medicine. Inducing hMSCs from human-induced pluripotent (hiPSCs), which have advantages autogenous no number issues, is highly valuable. However, current induction methods using FBS-containing culture medium problems, including immunogenicity, microbial contamination, low efficiency. To solve these we propose a chemically defined protocol incorporating transforming growth factor-beta 1 retinoic acid (RA) additives serum-free E6 for suspension embryoid bodies hiPSCs. Additionally, microgroove-patterned polydimethylsiloxane surfaces coated with temperature-sensitive N-isopropylacrylamide (PNIPAAm) were prepared efficient harvesting purification induced hiPSC-derived (hiPSC-MSCs). results showed that both supplementation RA patterned microgrooves width 20 μm could accelerate hiPSC-MSCs, realizing promising scalable production homogeneous cells. This study successfully established utilized to obtain clinically applicable show great promise tissue engineering, gene therapy, transplantation.

Language: Английский

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Engineering strong and tough collagen hydrogels and tissue constructs via twisting and crosslinking

Cell Reports Physical Science, Journal Year: 2025, Volume and Issue: unknown, P. 102454 - 102454

Published: Feb. 1, 2025

Language: Английский

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Wound Tissue Regeneration by Microfluidic Generated Fibroblast Cell/CuO Nanosheet-Loaded Alginate Hydrogel on an Excisional Full-Thickness Rat Model

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

Chronic ulcers present numerous challenges in treatment such as prolonged inflammation, infections resistant to drugs, and the formation of scars. In this research, we developed a calcium ion (Ca2+) cross-linked alginate (Alg) hydrogel loaded with CuO nanosheet/fibroblast cells via microfluidic system substantial efficiency accelerating healing preventing infection. Initially, soft lithography method was utilized fabricate system, which employed produce incorporating nanosheets copper oxide (CuO) MEF cells. The properties were analyzed by using FE-SEM, EDS/EDX, elemental mapping determine their physicochemical characteristics. viability mouse embryonic fibroblast (MEF) alginate-CuO explored through cell assay, antibacterial also studied colony-forming assay. abilities investigated an excisional, full-thickness wound rat model. Our results revealed proper antimicrobial angiogenic slight cytotoxicity for at concentration 25 μg/mL. alginate-CuO-cell-treated group exhibited faster contraction among all treatments. vivo assay along histology gene expression indicate synergistic cooperation between CuO, leading enhanced re-epithelialization, angiogenesis, matrix remodeling. therapeutic qualities like microbicidal, angiogenic, immune modulation, promotion ECM epithelium regeneration, resulting healing, developed. Moreover, there synergic impact noticed well improved blood vessels collagen accumulation. conclusion, biocompatible offers promising strategy effective without need invasive procedures.

Language: Английский

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Human Extracellular Matrix‐Like Collagen‐Based Hydrogels for Soft Tissue Regeneration and Mandibular Retrognathia Treatment

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract Skin aging, trauma, and congenital diseases lead to tissue defects functional loss. Effective regeneration remains challenging due limited material bioactivity inadequate consideration of mechanical cues. This study develops a hydrogel integrating human extracellular matrix‐like collagen (hCol) derived from induced adipose mesenchymal stem cells (hASCs) with hyaluronic acid (HA) polyethylene glycol diamine (PEGDA), engineered achieve modulus (≈1 kPa) representative subcutaneous soft tissue. The hCol, produced at scale, provides essential biochemical signals, which, in conjunction the properties, synergistically modulate immune responses, cellular differentiation, anti‐aging processes. Both animal experiments clinical trials validate regenerative efficacy hydrogels. Clinical therapy for mandibular retrognathia shows rapid sustained improvements, repair rates 95.2% 3 months 76.2% 6 months. These findings underscore hydrogel's ability reshape microenvironment, supporting immediate regeneration, while offering promising platform future biomaterial development engineering.

Language: Английский

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