Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract The advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐based genome editing technologies has opened up groundbreaking possibilities for treating a wide spectrum genetic disorders and diseases. However, the success these relies heavily on development efficient safe delivery systems. Among most promising approaches are natural synthetic nanocarrier‐mediated systems, including viral vectors, extracellular vesicles (EVs), engineered cellular membrane particles, liposomes, various nanoparticles. These carriers enhance efficacy CRISPR system by providing unique combination efficiency, specificity, reduced immunogenicity. Synthetic such as liposomes nanoparticles facilitate with high reproducibility customizable functions. Viral renowned their transduction efficiency broad tropism, serve powerful vehicles delivering components to cell types. EVs, RNA proteins, offer stealth mechanism evade immune detection, allowing targeted editors minimal off‐target effects. Engineered particles further improve simulating environment, enhancing uptake, minimizing response. This review explores innovative integration nanocarrier focusing recent advancements, applications, future directions in therapeutic editing.

Language: Английский

Nanosized Membrane Fusion-Triggered Three-Dimensional DNA Walker for Subtype-Specific Analysis of Breast Cancer Extracellular Vesicles

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Extracellular vesicles (EVs) are highly attractive in cancer diagnosis, owing to their ability reflect the molecular characteristics of source cells. Herein, we engineer a DNA-equipped liposome nanoprobe for developing nanosized membrane fusion-triggered three-dimensional (3D) DNA walker analysis breast EVs. Specifically, nanoprobes efficiently fuse with target EVs by recognizing surface-expressed epidermal growth factor receptors, thereby creating 3D tracks walker. Subsequently, walking probes targeting human receptor-2 (HER-2) were recruited onto fused manipulate walker, eventually generating considerable electrochemical signals. Results reveal good linearity between output and EV concentration also prove differentiate HER-2-positive patients monitor disease progression. Taken together, this work presents an effective approach early subtype-specific diagnosis cancer.

Language: Английский

Highly stable and near-infrared responsive phase change materials for targeted enzyme delivery toward cancer therapy

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101345 - 101345

Published: Nov. 19, 2024

Natural enzyme-based catalytic cascades have garnered increasing attention in cancer therapy, but their clinical utility is greatly limited due to loss of function during

Language: Английский