Ligation-Recognition Triggered RPA-Cas12a cis-cleavage Fluorogenic RNA Aptamer for One-pot and Label-free Detection of MicroRNA in Breast Cancer DOI
Haibo Liu,

Meng-Mei Lv,

Xiang Li

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: unknown, P. 117106 - 117106

Published: Dec. 1, 2024

Language: Английский

Programmable DNA Nanoswitch-Regulated Plasmonic CRISPR/Cas12a-Gold Nanostars Reporter Platform for Nucleic Acid and Non-Nucleic Acid Biomarker Analysis Assisted by a Spatial Confinement Effect DOI

Congkai Wang,

Xiaohan Xu, Yao Wang

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

CRISPR/Cas 12a system based nucleic acid and non-nucleic targets detection faces two challenges including (1) multiple crRNAs are needed for biomarkers (2) insufficient sensitivity resulted from photobleaching of fluorescent dyes the low kinetic cleavage rate a traditional single-strand (ssDNA) reporter. To address these limitations, we developed programmable DNA nanoswitch (NS)-regulated plasmonic CRISPR/Cas12a-gold nanostars (Au NSTs) reporter platform with assistance spatial confinement effect. Through simply programming target recognition sequence in NS, only one crRNA is required to detect both biomarkers. The limit decreased by ∼196-fold miRNA-375 122-fold prostate-specific antigen (PSA), respectively. Moreover, versatile evaluation PSA clinical urine samples can also be achieved, according which prostate cancer healthy groups well identified.

Language: Английский

Citations

1
A Programmable Automatic Cascade Machinery for Single-Molecule Profiling of Multiple Noncoding RNAs in Breast Tissues DOI
Li Wen, Wenjing Liu, Jun Lu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Noncoding RNAs (ncRNAs) are identified as critical regulatory molecules in tumorigenesis and progression. Investigating the expression patterns of multiple ncRNAs living cells tissues may facilitate diagnosis cancers. Herein, we develop a programmable automatic cascade machinery for single-molecule profiling ncRNAs. This method involves two successive amplification events that can convert extremely low-abundance target into abundant FAM/Cy5 generation amplified fluorescence signals. The subsequent detection identify piR-36026 with FAM signal DSCAM-AS1 Cy5 signal. Due to high efficiency signal-to-noise ratio imaging, this achieve sensitive limit 44.67 aM 45.71 DSCAM-AS1, it measure endogenous at single-cell level. Moreover, healthy breast cancer demonstrates feasibility proposed diagnostics. By programming recognition sequences dumbbell probes, be extended other cancer-related ncRNAs, great prospects clinical applications.

Language: Английский

Citations

0
Integration of biodegradable nanosphere-mediated spatial-localized cascade catalytic hairpin assembly with quantum dots for intracellular circular RNA imaging and clinical diagnosis DOI

Huimin Yuan,

Qi-qin Ge,

Jinping Hu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160685 - 160685

Published: Feb. 1, 2025

Language: Английский

Citations

0
A Brain-Targeting Nanoprobe for On-Site BACE1 Detection and Reversal of Microglia-Derived Neuroinflammation DOI
Xinyue Liu, Ruijie Cao, Yijing Dang

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Language: Английский

Citations

0
Recent advance in single-molecule detection and imaging DOI
Han Yun,

Weijie Tong,

Fei Ma

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118252 - 118252

Published: April 1, 2025

Language: Английский

Citations

0
Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary DOI
Lei Wang, Tingting Zhao,

Congkai Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Developing tumor cell-specific imaging approaches is essential for the clear delineation of margins. However, traditional suffered from low reaction kinetics as well limited specificity resulting their "always active" sensing mode, making it difficult to accurately depict boundary. To address these limitations, we developed an endogenous enzyme-activated spatial confinement DNA nanowire probe (E-SCNW) with enhanced tumor/normal cell discrimination ratio high precision cells The effect can improve kinetics, and enzyme-activation design confine fluorescence response region. Additionally, no additional delivery carriers were required during cross membrane into intracellular space. It worth noting that benefiting design, detection limit was decreased by nearly 25.6-fold 4.46-fold through using E-SCNW, indicating promising prospects in high-precision

Language: Английский

Citations

0
Unveiling the molecular cross-talk between piwi-interacting RNAs and steroid 5 alpha reductase type 2 in sperm dysfunction DOI Open Access

Adnan F. AL-Azaawie,

Ahmed AbdulJabbar Suleiman,

Mousa J. Mohammed

et al.

F&S Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Sequentially amplified integration of catalytic DNA circuits for high-performance intracellular imaging of miRNA and interpretation of mRNA-miRNA signalling pathway DOI

Mengdi Yu,

Yushi Wang,

Jinhua Shang

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 61, P. 102563 - 102563

Published: Dec. 1, 2024

Language: Английский

Citations

1
Ligation-Recognition Triggered RPA-Cas12a cis-cleavage Fluorogenic RNA Aptamer for One-pot and Label-free Detection of MicroRNA in Breast Cancer DOI
Haibo Liu,

Meng-Mei Lv,

Xiang Li

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: unknown, P. 117106 - 117106

Published: Dec. 1, 2024

Language: Английский

Citations

1