A Hybrid Plasmonic Nanoplatform for One-Pot DNA and Antibody Detection of Hepatitis B, Monkeypox, and Associated Infections

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Currently, nucleic acid and antibody tests for infectious diseases are conducted independently, often resulting in fragmented diagnostics delayed clinical decision-making. Given the diversity of analytes involved diseases, comprehensive detection is essential practice. Here, we introduce One-potNASH, a novel DNA hybrid plasmonic nanoplatform that unifies within single assay. By integrating extraction-free thermal lysis 1 μL serum, One-potNASH achieves single-DNA copy sensitivity while enhancing by more than 2 orders magnitude compared to an enzyme-linked immunosorbent Clinically, hepatitis B, monkeypox (MPX), associated infections can detect multiplexed antibodies (anti-HBc, anti-HCV, anti-HIV B anti-A29L, anti-H3L, MPX) with 100% specificity. This integrated approach addresses critical needs streamlining workflows, reducing diagnosis time, enabling evaluation thereby facilitating rapid therapeutic interventions improved patient outcomes.

Language: Английский

Advances in Microfluidic Single-Cell RNA Sequencing and Spatial Transcriptomics

Micromachines, Journal Year: 2025, Volume and Issue: 16(4), P. 426 - 426

Published: April 2, 2025

The development of micro- and nano-fabrication technologies has greatly advanced single-cell spatial omics technologies. With the advantages integration compartmentalization, microfluidic chips are capable generating high-throughput parallel reaction systems for screening analysis. As improve, can now integrate promising transcriptomics technologies, providing new insights from molecular characterization tissue gene expression profiles further revealing static even dynamic processes tissues in homeostasis disease. Here, we survey current landscape methods field multi-omics, as well assessing their relative limitations. We highlight how microfluidics been adapted improved to provide into multi-omics over past decade. Last, emphasize contributions microfluidic-based development, neuroscience, disease mechanisms, some perspectives technological advances translational clinical medicine.

Language: Английский

Combined Detection of Hepatitis B Virus Surface Antigen and Hepatitis B Virus DNA using DNA sensor

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(43), P. 7319 - 7324

Published: Jan. 1, 2024

A multi-detection strategy based on branched DNA nanostructures was developed to achieve the simultaneous high specificity identification and highly sensitive fluorescence responses HBV antigen DNA.

Language: Английский