Assembly of Matryoshka-Type Protein Nanocages for Compartmentalized Oxygen Sensing DOI
Wei Zhou,

S.-L. Ma,

Ruimin Gao

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Oxygen permeability is a critical property of protein nanocages (PNCs) that impacts or dictates the functions PNCs. However, it remains challenging to determine experimentally. Here, we report compartmentalized oxygen sensing inside PNCs by assembling matryoshka-type structures through interfacial engineering, namely, one PNC containing another smaller functionalized with small-molecule probes. in lumen outer can be probed conveniently via phosphorescence spectrometry. This method enabled analysis two representative PNCs, MS2 virus-like particles and Thermotoga maritima encapsulin, revealing former permeable, while latter impermeable. study establishes general approach for measuring shells, which provide an experimental basis understanding working mechanisms inspire applications like oxygen-sensitive oxygen-responsive sensing, catalysis, delivery. Also, tunable nested may serve as platforms designing hierarchical devices organelles.

Language: Английский

Assembly of Matryoshka-Type Protein Nanocages for Compartmentalized Oxygen Sensing DOI
Wei Zhou,

S.-L. Ma,

Ruimin Gao

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Oxygen permeability is a critical property of protein nanocages (PNCs) that impacts or dictates the functions PNCs. However, it remains challenging to determine experimentally. Here, we report compartmentalized oxygen sensing inside PNCs by assembling matryoshka-type structures through interfacial engineering, namely, one PNC containing another smaller functionalized with small-molecule probes. in lumen outer can be probed conveniently via phosphorescence spectrometry. This method enabled analysis two representative PNCs, MS2 virus-like particles and Thermotoga maritima encapsulin, revealing former permeable, while latter impermeable. study establishes general approach for measuring shells, which provide an experimental basis understanding working mechanisms inspire applications like oxygen-sensitive oxygen-responsive sensing, catalysis, delivery. Also, tunable nested may serve as platforms designing hierarchical devices organelles.

Language: Английский

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