Tutorial on impedance and dielectric spectroscopy for single-cell characterisation on microfluidic platforms: theory, practice, and recent advances

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Integration of low-frequency electrical impedance and broadband electromagnetic sensing with microfluidic devices enables high-throughput analysis cell size, membrane properties, intracellular characteristics.

Language: Английский

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Evaluation of Cancer Cell Invasion Ability Based on Electrochemical Impedance

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 91 - 91

Published: Feb. 6, 2025

Cancer metastasis is the leading cause of cancer-related deaths, with ability cancer cells to invade blood vessels or lymphatic systems, determining their metastatic potential. Therefore, rapid and accurate assessment cell invasiveness crucial. Current methods, such as Transwell assay fluorescent labeling, are complex, invasive, may disrupt physiological state live cells. In this study, we introduce an electrochemical impedance-based method for evaluating invasiveness, combining microfluidic technologies monitor invasion process in a dynamic environment. A stable chip 30 μm interdigital electrodes was developed, optimized HeLa detection. We identified 1 kHz optimal frequency achieving maximum impedance resolution invasiveness. By correlating response Zcells/Zno-cells established reliable model. This model validated hydrogen peroxide cytotoxicity assay, showing high correlation optical staining minimal error 1.89%, underscoring its potential drug efficacy prediction. The proposed offers detection, low cost, requires no manual intervention, making it efficient tool assessing therapeutic research.

Language: Английский

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Electric Field-Induced Effects in Eukaryotic Cells: Current Progress and Limitations

Tissue Engineering Part B Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Electric fields (EFs) offer a powerful tool for manipulating cells and modulating their behavior, holding significant promise regenerative medicine cell biology. We provide comprehensive overview of the effects different types EF on eukaryotic with special focus physical mechanisms signaling pathways involved. Direct current induces electrophoresis electroosmosis, influencing migration, proliferation, differentiation. Alternating EF, through dielectric polarization dielectrophoresis, enables manipulation, trapping, sorting. Pulsed particularly high-intensity, short-duration pulses, reversible irreversible electroporation, facilitating drug gene delivery. The review covers some technological aspects generation, emphasizing importance experimental setups, integration microfluidic platforms high-throughput analysis precise manipulations. Furthermore, synergistic potential combining EFs optical tweezers is highlighted, enabling fine-tuned control positioning, intercellular interactions, measurement biophysical properties. Finally, addresses limitations application, such as field heterogeneity side effects, outlines directions future studies, including developing minimally invasive delivery methods.

Language: Английский

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A Gravity-driven Microfluidic Metering System for Automation of Multiplexed Bioassays

Lab on a Chip, Journal Year: 2024, Volume and Issue: 25(2), P. 175 - 186

Published: Dec. 5, 2024

Our gravity-driven microfluidic metering system enables automated multiplexed fluid metering, multistep control, and multi-chamber signal readout.

Language: Английский

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