Interplay of Matrix Stiffness and Cell–Cell Contact in Regulating Differentiation of Stem Cells DOI
Kai Ye,

Luping Cao,

Shiyu Li

et al.

ACS Applied Materials & Interfaces, Journal Year: 2015, Volume and Issue: 8(34), P. 21903 - 21913

Published: Nov. 24, 2015

Stem cells are capable of sensing and responding to the mechanical properties extracellular matrixes (ECMs). It is well-known that, while osteogenesis promoted on stiff matrixes, adipogenesis enhanced soft ones. Herein, we report an "abnormal" tendency matrix-stiffness-directed stem cell differentiation. Well-defined nanoarrays cell-adhesive arginine-glycine-aspartate (RGD) peptides were modified onto surfaces persistently nonfouling poly(ethylene glycol) (PEG) hydrogels achieve controlled specific adhesion simultaneously eliminate nonspecific protein adsorption. Mesenchymal cultivated RGD-nanopatterned PEG with same RGD nanospacing but different hydrogel stiffnesses incubated in induction medium examine effect matrix stiffness osteogenic adipogenic differentiation extents. When kept at a low density during period, was consistent previous reports literature; however, both lineage commitments favored matrices high density. We interpreted such complicated two-dimensional culture as interplay cell–cell contact. As result, this study strengthens essence highlights combinatory effects ECM cues

Language: Английский

Mechanical forces direct stem cell behaviour in development and regeneration DOI
Kyle H. Vining, David Mooney

Nature Reviews Molecular Cell Biology, Journal Year: 2017, Volume and Issue: 18(12), P. 728 - 742

Published: Nov. 8, 2017

Language: Английский

Citations

1331
The stiffness of living tissues and its implications for tissue engineering DOI
Carlos F. Guimarães, Luca Gasperini, Alexandra P. Marques

et al.

Nature Reviews Materials, Journal Year: 2020, Volume and Issue: 5(5), P. 351 - 370

Published: Feb. 21, 2020

Language: Английский

Citations

1153
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment DOI
Guoyou Huang, Fei Li, Xin Zhao

et al.

Chemical Reviews, Journal Year: 2017, Volume and Issue: 117(20), P. 12764 - 12850

Published: Oct. 9, 2017

The cell microenvironment has emerged as a key determinant of behavior and function in development, physiology, pathophysiology. extracellular matrix (ECM) within the serves not only structural foundation for cells but also source three-dimensional (3D) biochemical biophysical cues that trigger regulate behaviors. Increasing evidence suggests 3D character is required development many critical responses observed vivo, fueling surge functional biomimetic materials engineering microenvironment. Progress design such improved control behaviors advanced fields tissue regeneration, vitro models, large-scale differentiation, immunotherapy, gene therapy. However, field still its infancy, discoveries about nature cell–microenvironment interactions continue to overturn much early progress field. Key challenges be dissecting roles chemistry, structure, mechanics, electrophysiology microenvironment, understanding harnessing periodicity drift these factors. This review encapsulates where recent advances appear leave ever-shifting state art, it highlights areas which substantial potential uncertainty remain.

Language: Английский

Citations

687
The Horizon of Materiobiology: A Perspective on Material-Guided Cell Behaviors and Tissue Engineering DOI
Yulin Li, Yin Xiao,

Changsheng Liu

et al.

Chemical Reviews, Journal Year: 2017, Volume and Issue: 117(5), P. 4376 - 4421

Published: Feb. 21, 2017

Although the biological functions of cell and tissue can be regulated by biochemical factors (e.g., growth factors, hormones), biophysical effects materials on regulation activity are receiving more attention. In this Review, we systematically summarize recent progress how biomaterials with controllable properties compositional/degradable dynamics, mechanical properties, 2D topography, 3D geometry) regulate behaviors adhesion, spreading, proliferation, alignment, differentiation or self-maintenance stem cells) tissue/organ functions. How features influence regeneration have been elucidated. Current challenges a perspective development novel that modulate specific discussed. The interdependent relationship between biology leads us to propose concept "materiobiology", which is scientific discipline studies at cell, tissue, organ, whole organism levels. This Review highlights it important develop ECM-mimicking having self-regenerative capacity stimulate regeneration, instead attempting recreate complexity living tissues constructs ex vivo. principles materiobiology may benefit providing combinative bioactive cues activate migration cells from endogenous reservoirs (i.e., niches), robust scalable self-healing mechanisms, unlock body's innate powers regeneration.

Language: Английский

Citations

511
Cellulose Biomaterials for Tissue Engineering DOI Creative Commons

Ryan J. Hickey,

Andrew E. Pelling

Frontiers in Bioengineering and Biotechnology, Journal Year: 2019, Volume and Issue: 7

Published: March 22, 2019

In this review, we highlight the importance of nanostructure cellulose-based biomaterials to allow cellular adhesion, contribution macroscale mechanical properties, and several key applications these materials for fundamental scientific research biomedical engineering. Different features on nanoscale can have impacts tissue function. Cellulose is a diverse material with tunable properties promising platform biomaterial development Cellulose-based offer some important advantages over conventional synthetic materials. Here provide an up-to-date summary status field in context bottom-up approaches We anticipate that will continue expand because diversity versatility biochemical biophysical characteristics highlighted review.

Language: Английский

Citations

386
Cell–extracellular matrix mechanotransduction in 3D DOI
Aashrith Saraswathibhatla, Dhiraj Indana, Ovijit Chaudhuri

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(7), P. 495 - 516

Published: Feb. 27, 2023

Language: Английский

Citations

335
Engineered materials for organoid systems DOI
Michael J. Kratochvil, Alexis J. Seymour, Thomas L. Li

et al.

Nature Reviews Materials, Journal Year: 2019, Volume and Issue: 4(9), P. 606 - 622

Published: Aug. 16, 2019

Language: Английский

Citations

330
Biological responses to physicochemical properties of biomaterial surface DOI Creative Commons
Maryam Rahmati, Eduardo A. Silva, Janne E. Reseland

et al.

Chemical Society Reviews, Journal Year: 2020, Volume and Issue: 49(15), P. 5178 - 5224

Published: Jan. 1, 2020

Biomedical scientists use chemistry-driven processes found in nature as an inspiration to design biomaterials promising diagnostic tools, therapeutic solutions, or tissue substitutes.

Language: Английский

Citations

256
Stimuli-Responsive, Pentapeptide, Nanofiber Hydrogel for Tissue Engineering DOI
James D. Tang, Cameron Mura, Kyle J. Lampe

et al.

Journal of the American Chemical Society, Journal Year: 2019, Volume and Issue: 141(12), P. 4886 - 4899

Published: March 4, 2019

Short peptides are uniquely versatile building blocks for self-assembly. Supramolecular peptide assemblies can be used to construct functional hydrogel biomaterials-an attractive approach neural tissue engineering. Here, we report a new class of short, five-residue that form hydrogels with nanofiber structures. Using rheology and spectroscopy, describe how sequence variations, pH, concentration alter the mechanical properties our pentapeptide hydrogels. We find this seven unmodified forms robust from 0.2-20 kPa at low weight percent (less than 3 wt %) in cell culture media undergoes shear-thinning rapid self-healing. The self-assemble into long fibrils sequence-dependent fibrillar morphologies. These exhibit unique twisted ribbon shape, as visualized by transmission electron microscopy (TEM) Cryo-EM imaging, diameters tens nanometers periodicities similar amyloid fibrils. Experimental gelation behavior corroborates molecular dynamics simulations, which demonstrate assembly behavior, an increase β-sheet content, patterns variation solvent accessibility. Our rapidly assembling pentapeptides injectable delivery (RAPID) syringe-injectable support cytocompatible encapsulation oligodendrocyte progenitor cells (OPCs), well their proliferation three-dimensional process extension. Furthermore, RAPID gels protect OPCs membrane disruption acute loss viability when ejected syringe needle, highlighting protective capability potential carriers transplantation therapies. tunable structural these supramolecular shown permissive expansion remodeling, making system suitable material engineering applications.

Language: Английский

Citations

255
3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate DOI
Yufei Ma, Min Lin, Guoyou Huang

et al.

Advanced Materials, Journal Year: 2018, Volume and Issue: 30(49)

Published: July 31, 2018

Abstract Stem cells hold great promise for widespread biomedical applications, which stem cell fate needs to be well tailored. Besides biochemical cues, accumulating evidence has demonstrated that spatiotemporal biophysical cues (especially mechanical cues) imposed by microenvironments also critically impact on the fate. As such, various biomaterials, especially hydrogels due their tunable physicochemical properties and advanced fabrication approaches, are developed spatiotemporally manipulate in vitro so as recapitulate 3D microenvironment where reside vivo. Here, main experience native summarized. Then, recent advances design of hydrogel materials with engineering highlighted. These engineered crucial guiding potential applications subsequently discussed. Finally, challenges future perspectives presented.

Language: Английский

Citations

199