A new portable toluidine blue/aptamer complex-on-polyethyleneimine-coated gold nanoparticles-based sensor for label-free electrochemical detection of alpha-fetoprotein DOI Creative Commons
Patrawadee Yaiwong,

Siriporn Anuthum,

Padchanee Sangthong

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: May 22, 2023

The quantification of alpha-fetoprotein (AFP) as a potential liver cancer biomarker which is generally found in ultratrace level significance biomedical diagnostics. Therefore, it challenging to find strategy fabricate highly sensitive electrochemical device towards AFP detection through electrode modification for signal generation and amplification. This work shows the construction simple, reliable, sensitive, label-free aptasensor based on polyethyleneimine-coated gold nanoparticles (PEI-AuNPs). A disposable ItalSens screen-printed (SPE) employed fabricating sensor by successive modifying with PEI-AuNPs, aptamer, bovine serum albumin (BSA), toluidine blue (TB), respectively. assay easily performed when inserted into small Sensit/Smart potentiostat connected smartphone. readout derives from response TB intercalating aptamer-modified after binding target. decrease current proposed proportional concentration due restriction electron transfer pathway number insulating AFP/aptamer complexes surface. PEI-AuNPs improve SPE's reactivity provide large surface area aptamer immobilization whereas provides selectivity target AFP. Consequently, this biosensor selective analysis. developed reveals linear range 10 50000 pg mL-1 R2 = 0.9977 provided limit (LOD) 9.5 human serum. With its simplicity robustness, anticipated that electrochemical-based will be benefit clinical diagnosis further other biomarkers

Language: Английский

Fundamentals of bio-electrochemical sensing DOI Creative Commons
Mahesh M. Shanbhag, G. Manasa, Ronald J. Mascarenhas

et al.

Chemical Engineering Journal Advances, Journal Year: 2023, Volume and Issue: 16, P. 100516 - 100516

Published: June 7, 2023

The utilization of bio-functionalities such as biorecognition or catalysis derived them their name biosensors. Bio-electrochemical sensing is a new discipline that combines the advantages biological detection and electrochemical transduction. sensors are devices use materials enzymes, antibodies, DNA, cells receptors to detect target analytes in variety samples. Electrodes convert interactions into electrical impulses, which can be studied using various techniques. have demonstrated significant promise for clinical diagnostics, environmental monitoring, food safety, biotechnology. Biosensors received numerous applications recent years because they fast, simple, inexpensive practical applications. In this article, we cover basic principles, design strategies, immobilization regeneration techniques, along with bio-electrochemical sensors. Finally, article discusses rationale developing biosensors context bio-receptors applied.

Language: Английский

Citations

107
Highly Sensitive Electrochemical Immunosensor Platforms for Dual Detection of SARS-CoV-2 Antigen and Antibody based on Gold Nanoparticle Functionalized Graphene Oxide Nanocomposites DOI Open Access
Mohd. Abubakar Sadique, Shalu Yadav, Pushpesh Ranjan

et al.

ACS Applied Bio Materials, Journal Year: 2022, Volume and Issue: 5(5), P. 2421 - 2430

Published: May 6, 2022

In this work, we report a facile synthesis of graphene oxide-gold (GO-Au) nanocomposites by electrodeposition. The fabricated electrochemical immunosensors are utilized for the dual detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen and SARS-CoV-2 antibody. GO-Au has been characterized UV-vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), cyclic voltammetry (CV), differential pulse (DPV), impedance spectroscopy (EIS) its biosensing properties. linear range immunosensor is 10.0 ag mL-1 to 50.0 ng mL-1, whereas that antibody ranges from 1.0 fg mL-1. calculated limit (LOD) 3.99 with high sensitivity. validation also carried out on patient serum swab samples COVID-19 patients. results suggest successful utilization very low enabling use in clinical samples. Further work needed standardization translation screen-printed electrodes portable commercial applications.

Language: Английский

Citations

72
Recent Advances in Electrochemical Immunosensors with Nanomaterial Assistance for Signal Amplification DOI Creative Commons
Avinash V. Police Patil, Yu-Sheng Chuang, Chenzhong Li

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(1), P. 125 - 125

Published: Jan. 11, 2023

Electrochemical immunosensors have attracted immense attention due to the ease of mass electrode production and high compatibility miniature electric reader, which is beneficial for developing point-of-care diagnostic devices. can be divided into label-free label-based sensing strategies equipped with potentiometric, amperometric, voltammetric, or impedimetric detectors. Emerging nanomaterials are frequently used on electrochemical as a highly rough conductive interface electrodes nanocarriers immobilizing capture antibodies, electroactive mediators, catalyzers. Adopting increase immunosensor characteristics lower detection limits better sensitivity. Recent research has shown innovative immobilization procedures meet requirements different immunosensors. This review discusses past five years advances in (metal nanoparticles, metal nanostructures, carbon nanotubes, graphene) integrated immunosensor. Furthermore, new tendency endeavors nanomaterial-based discussed.

Language: Английский

Citations

55
An electrochemical aptasensor based on g-C3N4/Fe3O4/PANI nanocomposite applying cancer antigen_125 biomarkers detection DOI
Amin Foroozandeh, Majid Abdouss,

Hossein SalarAmoli

et al.

Process Biochemistry, Journal Year: 2023, Volume and Issue: 127, P. 82 - 91

Published: Feb. 10, 2023

Language: Английский

Citations

43
Development of a labeled-free and labeled electrochemical aptasensor for the detection of cancer antigen 125 by using magnetic g-C3N4/MoS2 nanocomposite DOI Creative Commons
Amin Foroozandeh,

Hossein SalarAmoli,

Majid Abdouss

et al.

Sensors and Actuators Reports, Journal Year: 2024, Volume and Issue: 7, P. 100195 - 100195

Published: April 24, 2024

Efficient and timely detection of cancer biomarkers is pivotal for enhancing treatment outcomes mitigating patient mortality. This study addresses the pressing need a swift, accurate, non-invasive method to identify antigen 125 (CA125), vital biomarker in ovarian cancer. Leveraging growing prominence nano-biosensors their high selectivity sensitivity, we present development characterization an innovative electrochemical nano-biosensor. The sensor, featuring aptamer strands immobilized on glassy carbon electrode modified with graphitic nitrides, molybdenum disulfide, magnetic nanoparticles (g-C3N4/MoS2/Fe3O4), demonstrates superior sensitivity accuracy CA125 detection. Utilizing methylene blue labeled ferrocyanide label-free detection, our aptasensor achieves low limit (LOD) at 0.202 U.mL−1 0.215 U.mL−1, respectively, broad range from 2 10 U.mL−1. exhibits pronounced affinity CA125, demonstrating enhanced stability compared other biomolecules. Crucially, evaluation both normal serum samples underscores aptasensor's remarkable performance. These findings not only establish robust foundation future research diagnosis but also highlight potential clinical impact nano-biosensor advancing early methodologies.

Language: Английский

Citations

21
A label-free electrochemical immunosensor for rapid detection of salmonella in milk by using CoFe-MOFs-graphene modified electrode DOI

Kaiwen Feng,

Ting Li, Cuizhu Ye

et al.

Food Control, Journal Year: 2021, Volume and Issue: 130, P. 108357 - 108357

Published: June 19, 2021

Language: Английский

Citations

69
Functionalized Graphene Platforms for Anticancer Drug Delivery DOI Creative Commons

Shabnam Sattari,

Mohsen Adeli, Siamak Beyranvand

et al.

International Journal of Nanomedicine, Journal Year: 2021, Volume and Issue: Volume 16, P. 5955 - 5980

Published: Aug. 1, 2021

Two-dimensional nanomaterials are emerging as promising candidates for a wide range of biomedical applications including tissue engineering, biosensing, pathogen incapacitation, wound healing, and gene drug delivery. Graphene, due to its high surface area, photothermal property, loading capacity, efficient cellular uptake, is at the forefront these materials plays key role in this multidisciplinary research field. Poor water dispersibility low functionality graphene, however, hamper hybridization into new nanostructures future nanomedicine. Functionalization either by covalent or non-covalent methods, most useful strategy improve dispersion well processability devices. In review, recent advances functionalization graphene derivatives different (macro)molecules reported explained. particular, hydrophilic oxide (GO) their physicochemical properties discussed. We have focused on anticancer delivery polyfunctional sheets.

Language: Английский

Citations

69
Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application DOI

Thangapandi Kalyani,

Arumugam Sangili, Amalesh Nanda

et al.

Bioelectrochemistry, Journal Year: 2021, Volume and Issue: 139, P. 107740 - 107740

Published: Jan. 14, 2021

Language: Английский

Citations

66
Fabrication of label-free and ultrasensitive electrochemical immunosensor based on molybdenum disulfide nanoparticles modified disposable ITO: An analytical platform for antibiotic detection in food samples DOI
Amit K. Yadav, Damini Verma,

G.B.V.S. Lakshmi

et al.

Food Chemistry, Journal Year: 2021, Volume and Issue: 363, P. 130245 - 130245

Published: May 29, 2021

Language: Английский

Citations

66
Graphene Biodevices for Early Disease Diagnosis Based on Biomarker Detection DOI
Qingfang Han, Jinbo Pang, Yufen Li

et al.

ACS Sensors, Journal Year: 2021, Volume and Issue: 6(11), P. 3841 - 3881

Published: Oct. 26, 2021

The early diagnosis of diseases plays a vital role in healthcare and the extension human life. Graphene-based biosensors have boosted by detecting monitoring related biomarkers, providing better understanding various physiological pathological processes. They generated tremendous interest, made significant advances, offered promising application prospects. In this paper, we discuss background graphene biosensors, including properties functionalization biosensors. Second, technologies adopted are discussed, such as field-effect transistors electrochemical optical methods. Subsequently, highlight for ions, small molecules, macromolecules, viruses, bacteria, living cells. Finally, opportunities challenges graphene-based broad research interests discussed.

Language: Английский

Citations

63