Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
11
Published: May 22, 2023
The
quantification
of
alpha-fetoprotein
(AFP)
as
a
potential
liver
cancer
biomarker
which
is
generally
found
in
ultratrace
level
significance
biomedical
diagnostics.
Therefore,
it
challenging
to
find
strategy
fabricate
highly
sensitive
electrochemical
device
towards
AFP
detection
through
electrode
modification
for
signal
generation
and
amplification.
This
work
shows
the
construction
simple,
reliable,
sensitive,
label-free
aptasensor
based
on
polyethyleneimine-coated
gold
nanoparticles
(PEI-AuNPs).
A
disposable
ItalSens
screen-printed
(SPE)
employed
fabricating
sensor
by
successive
modifying
with
PEI-AuNPs,
aptamer,
bovine
serum
albumin
(BSA),
toluidine
blue
(TB),
respectively.
assay
easily
performed
when
inserted
into
small
Sensit/Smart
potentiostat
connected
smartphone.
readout
derives
from
response
TB
intercalating
aptamer-modified
after
binding
target.
decrease
current
proposed
proportional
concentration
due
restriction
electron
transfer
pathway
number
insulating
AFP/aptamer
complexes
surface.
PEI-AuNPs
improve
SPE's
reactivity
provide
large
surface
area
aptamer
immobilization
whereas
provides
selectivity
target
AFP.
Consequently,
this
biosensor
selective
analysis.
developed
reveals
linear
range
10
50000
pg
mL-1
R2
=
0.9977
provided
limit
(LOD)
9.5
human
serum.
With
its
simplicity
robustness,
anticipated
that
electrochemical-based
will
be
benefit
clinical
diagnosis
further
other
biomarkers
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(1), P. 99 - 99
Published: Jan. 6, 2023
Nowadays,
diagnosing
early-stage
cancers
can
be
vital
for
saving
patients
and
dramatically
decreases
mortality
rates.
Therefore,
specificity
sensitivity
in
the
detection
of
cancer
antigens
should
elaborately
ensured.
Some
diagnosed
via
detecting
antigen
CA-125,
such
as
ovarian
cancer,
required
treatments
applied
more
efficiently.
Thus,
CA-125
by
employing
various
optical
or
electrochemical
biosensors
is
a
preliminary
crucial
step
to
treating
cancers.
In
this
review,
diverse
range
means
are
reviewed.
Furthermore,
an
applicable
comparison
their
performance
provided,
several
commercial
kits
investigated,
applications
compared
discussed
determine
whether
they
accurate
enough.
Hybrid Advances,
Journal Year:
2023,
Volume and Issue:
5, P. 100123 - 100123
Published: Dec. 12, 2023
Graphene
oxide
based
nanomaterials
i.e.
graphene
(GO),
reduced
GO,
are
being
researched
extensively
owing
to
their
fascinating
as
well
promising
structural,
conducting,
electrical
and
other
physiochemical
properties.
Incorporating
GO/rGO
into
various
matrices
by
chemical
physical
processes
results
in
nanocomposites
with
remarkable
This
review
provides
a
detailed
discussion
on
combining
GO
conducting
polymers,
noble
metals,
metal
oxides,
functional
improve
the
analytical
performance
electrochemical
detection
platforms.
The
use
of
such
GO/rGO-modified
materials
for
early
biomarker
which
facilitates
treatment,
reduces
mortality,
improves
patient
survival
rates.
A
Wide
range
biosensing
methodologies
biomarkers
serious
onset
have
been
discussed
diabetes,
hypertension,
cancer,
neurotransmitters,
stroke,
bacterial
viral
infections
utilizing
GO/rGO-based
nanomaterials.
synthetic
methodology,
mechanistic
pathway
immunosensing
that
insights
device
fabrication
future
researchers
also
highlighted.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
11
Published: May 22, 2023
The
quantification
of
alpha-fetoprotein
(AFP)
as
a
potential
liver
cancer
biomarker
which
is
generally
found
in
ultratrace
level
significance
biomedical
diagnostics.
Therefore,
it
challenging
to
find
strategy
fabricate
highly
sensitive
electrochemical
device
towards
AFP
detection
through
electrode
modification
for
signal
generation
and
amplification.
This
work
shows
the
construction
simple,
reliable,
sensitive,
label-free
aptasensor
based
on
polyethyleneimine-coated
gold
nanoparticles
(PEI-AuNPs).
A
disposable
ItalSens
screen-printed
(SPE)
employed
fabricating
sensor
by
successive
modifying
with
PEI-AuNPs,
aptamer,
bovine
serum
albumin
(BSA),
toluidine
blue
(TB),
respectively.
assay
easily
performed
when
inserted
into
small
Sensit/Smart
potentiostat
connected
smartphone.
readout
derives
from
response
TB
intercalating
aptamer-modified
after
binding
target.
decrease
current
proposed
proportional
concentration
due
restriction
electron
transfer
pathway
number
insulating
AFP/aptamer
complexes
surface.
PEI-AuNPs
improve
SPE's
reactivity
provide
large
surface
area
aptamer
immobilization
whereas
provides
selectivity
target
AFP.
Consequently,
this
biosensor
selective
analysis.
developed
reveals
linear
range
10
50000
pg
mL-1
R2
=
0.9977
provided
limit
(LOD)
9.5
human
serum.
With
its
simplicity
robustness,
anticipated
that
electrochemical-based
will
be
benefit
clinical
diagnosis
further
other
biomarkers
