Current trends and future potential in the detection of avian coronaviruses: An emphasis on sensors-based technologies.

Virology, Journal Year: 2025, Volume and Issue: 604, P. 110399 - 110399

Published: Jan. 18, 2025

Infectious bronchitis virus (IBV), an avian coronavirus, member of the genus Gammacoronavirus, poses significant threats to poultry health, causing severe respiratory, reproductive, and renal infections. The genetic diversity IBV, driven by mutations, recombination deletions, has led emergence numerous serotypes genotypes, complicating both diagnosis control measures. Rapid accurate diagnostic tools are essential for effective disease management minimizing economic losses. Conventional methods, such as PCR, isolation, serological assays, hindered limitations in sensitivity, specificity, turnaround time. In contrast, innovative biosensor platforms employing advanced detection mechanisms-including electrochemical, optical, piezoelectric sensors-offer a transformative solution. These technologies provide portable, highly sensitive, rapid IBV detection. Beyond addressing challenges conventional these biosensor-based approaches facilitate real-time monitoring enhance surveillance. This review highlights potential biosensors their integration into strategies coronavirus infections, presenting them promising alternative precise efficient

Language: Английский

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Use of Quantum Dots as Nanotheranostic Agents: Emerging Applications in Rare Genetic Diseases

Small, Journal Year: 2025, Volume and Issue: 21(9)

Published: Jan. 19, 2025

Abstract Rare genetic diseases (RGDs) affect a small percentage of the global population but collectively have substantial impact due to their diverse manifestations. Although precise reasons behind these remain unclear, roughly 80% cases are genetically linked. Recent efforts focus on understanding pathology and developing new diagnostic therapeutic approaches for RGDs. However, there persists gap between fundamental research clinical approaches, where advancements in nanotechnology offer promising improvements. In this context, nanosized light‐emitting quantum dots (QDs), ranging from 2–10 nm, materials applications. Their size‐tunable light emission, high yield, photostability allow tracking cargo. Additionally, QDs can be functionalized with agents, antibodies, or peptides target specific cellular pathways, enhancing treatment efficacy while minimizing side effects. By combining capabilities single platform, thus versatile powerful approach tackle rare disorders. Despite several reviews various applications QDs, utilization domain RGDs is not well documented. This review highlight QDs’ potential diagnosing treating certain addresses challenges limiting application.

Language: Английский

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Highly‐Specific Single‐Stranded Oligonucleotides and Functional Nanoprobes for Clinical Determination of Chlamydia Trachomatis and Neisseria Gonorrhoeae Infections

Advanced Science, Journal Year: 2023, Volume and Issue: 10(36)

Published: Oct. 23, 2023

Abstract Early detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoe ae (NG) is the key to controlling spread these bacterial infections. An important step in developing biosensors involves identifying reliable sensing probes against specific genetic targets for CT NG. Here, authors have designed single‐stranded oligonucleotides (ssDNAs) targeting mutually conserved regions cryptic plasmid chromosomal DNA both The 5′‐ 3′‐ ends ssDNAs are differentially functionalized with thiol groups coupled gold nanoparticles (AuNP) develop absorbance‐based assay. AuNPs agglomerate selectively presence its target sequence demonstrate a change their surface plasmon resonance. optimized assay then used detect NG extracted from 60 anonymized clinical samples sensitivity ∼100%. limit assays found be 7 5 copies/µL respectively. Furthermore, it can successfully levels two bacteria without need extraction via lateral flow‐based platform. These thus hold potential employed clinics rapid efficient monitoring sexually transmitted

Language: Английский

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Cooperative effect of complementary antisense oligonucleotides and CRISPR effectors for universal DNA-based pathogen assay using nano-enabled colorimetry

Materials Today, Journal Year: 2024, Volume and Issue: 80, P. 374 - 394

Published: Oct. 12, 2024

Language: Английский

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Recent advances in electrochemical aptasensors and genosensors for the detection of pathogens

Environmental Research, Journal Year: 2023, Volume and Issue: 243, P. 117850 - 117850

Published: Dec. 9, 2023

Language: Английский

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