Ecofriendly and Conductive Corn-Cob-Based Biomass Driven from Aqueous Deep Eutectic Solvents: A Suitable Media for Enzyme Structure, Storage, and Activity at High Temperature DOI
Niketa Yadav, Deepak Chahar, Meena Bisht

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(46), P. 16884 - 16895

Published: Nov. 6, 2024

Production of biomaterials from lignocellulosic biomass waste is prime importance for diverse applications. However, the use harsh chemicals, extreme conditions, and tedious processes to convert it into useful materials are major bottlenecks its application. Herein, leftover corn-cob (CB) was converted functional carbonaceous material via a solvothermal process using water in deep eutectic solvents (DESs) by employing two DESs namely, choline chloride-glycerol (ChCl-Gly) ChCl-ethylene glycol (ChCl-EG). The synthesized CB-based carbon materials, CB-ChCl-Gly CB-ChCl-EG, were characterized verify incorporated morphological variations. obtained results showed that rich oxygen functionality with more porous, globular surface structure. Further, detailed analysis feasibility as biocompatible carriers cytochrome-c (Cyt-c) evaluated. peroxidase-like activity remarkably enhanced up 1.5-fold compared native enzyme. biocompatibility these validated Michaelis–Menten equation. Besides this, time- temperature-dependent studies Cyt-c after immobilization biomass-based demonstrated preservation structural integrity well improved enzymatic activity. Various biophysical techniques secondary tertiary structures enzymes immobilization. micrograph transmission electron microscopy (TEM) hydrodynamic size (dH) zeta potential values measured dynamic light scattering (DLS) successful protein on materials. Interestingly, structure maintained at higher temperatures presence both CB-ChCl-EG Overall, this study elucidates sustainable source ecofriendly method preparation displays beneficial influence stability Cyt-c. Remarkably, proving be protein-friendly host, CB will helpful long-term packing, which may provide foundation imaging modalities biomolecules industrial

Language: Английский

Interactions of Polydopamine Nanoparticles with Serine and Cysteine Proteases: Implications for Enhancing Protein Stabilization and Enzyme Activity DOI
Deepak Chahar,

Bindu Yadav,

Pannuru Venkatesu

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(22), P. 26202 - 26214

Published: Nov. 7, 2024

The interactions between polydopamine nanoparticles (PDA NPs) with serine protease α-chymotrypsin (α-CT) and cysteine stem bromelain (SB) have prompted significant emphasis due to their potential implications in biocatalysis therapeutic applications. This study addresses the structural integrity, binding kinetics, catalytic of α-CT SB presence PDA NPs by employing a combination spectroscopic techniques thermodynamic kinetic analyses. results reveal that bind both enzymes (α-CT SB) create stable complex without disrupting active sites. Furthermore, fluorescence, circular dichroism (CD), dynamic light scattering (DLS) analysis clearly demonstrate is strong compared + NPs. data implies significantly enhance thermal stability SB, as evidenced increased transition temperature (Tm) 2.68 ± 0.50 °C 3.35 SB. Interestingly, ζ-potential microscopic such TEM FESEM pointed toward adsorption proteases over surface Additionally, spectrophotometric assay studies confirm retention enzyme activity upon conjugation Further, (Vmax, Km) (ΔGu, ΔHm, ΔSm, ΔCp) parameters also concluded formation enzyme–NP complex. conveys developing advanced enzyme–NPs integrated for enhancing protein diverse biomedical industrial

Language: Английский

Citations

1
Ecofriendly and Conductive Corn-Cob-Based Biomass Driven from Aqueous Deep Eutectic Solvents: A Suitable Media for Enzyme Structure, Storage, and Activity at High Temperature DOI
Niketa Yadav, Deepak Chahar, Meena Bisht

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(46), P. 16884 - 16895

Published: Nov. 6, 2024

Production of biomaterials from lignocellulosic biomass waste is prime importance for diverse applications. However, the use harsh chemicals, extreme conditions, and tedious processes to convert it into useful materials are major bottlenecks its application. Herein, leftover corn-cob (CB) was converted functional carbonaceous material via a solvothermal process using water in deep eutectic solvents (DESs) by employing two DESs namely, choline chloride-glycerol (ChCl-Gly) ChCl-ethylene glycol (ChCl-EG). The synthesized CB-based carbon materials, CB-ChCl-Gly CB-ChCl-EG, were characterized verify incorporated morphological variations. obtained results showed that rich oxygen functionality with more porous, globular surface structure. Further, detailed analysis feasibility as biocompatible carriers cytochrome-c (Cyt-c) evaluated. peroxidase-like activity remarkably enhanced up 1.5-fold compared native enzyme. biocompatibility these validated Michaelis–Menten equation. Besides this, time- temperature-dependent studies Cyt-c after immobilization biomass-based demonstrated preservation structural integrity well improved enzymatic activity. Various biophysical techniques secondary tertiary structures enzymes immobilization. micrograph transmission electron microscopy (TEM) hydrodynamic size (dH) zeta potential values measured dynamic light scattering (DLS) successful protein on materials. Interestingly, structure maintained at higher temperatures presence both CB-ChCl-EG Overall, this study elucidates sustainable source ecofriendly method preparation displays beneficial influence stability Cyt-c. Remarkably, proving be protein-friendly host, CB will helpful long-term packing, which may provide foundation imaging modalities biomolecules industrial

Language: Английский

Citations

0